LOCUS       sequence-158457-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that expresses Cas9C in mammalian cells; co-expresses 
            turboGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pSMVP-Cas9C-P2A-turboGFP
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             631..642
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agccgctagc gctaccggtc gccaccatgg cggcggcgtg cccggaactg cgtcagctgg
       61 cgcagagcga tgtgtattgg gatccgattg tgagcattga accggatggc gtggaagaag
      121 tgtttgatct gaccgtgccg ggcccgcata actttgtggc gaacgatatt attgcgcata
      181 actctggcca gggggacagt cttcacgagc acatcgctaa tcttgcaggt agcccagcta
      241 tcaaaaaggg aatactgcag accgttaagg tcgtggatga actcgtcaaa gtaatgggaa
      301 ggcataagcc cgagaatatc gttatcgaga tggcccgaga gaaccaaact acccagaagg
      361 gacagaagaa cagtagggaa aggatgaaga ggattgaaga gggtataaaa gaactggggt
      421 cccaaatcct taaggaacac ccagttgaaa acacccagct tcagaatgag aagctctacc
      481 tgtactacct gcagaacggc agggacatgt acgtggatca ggaactggac atcaatcggc
      541 tctccgacta cgacgtggat catatcgtgc cccagtcttt tctcaaagat gattctattg
      601 ataataaagt gttgacaaga tccgataaaa atagagggaa gagtgataac gtcccctcag
      661 aagaagttgt caagaaaatg aaaaattatt ggcggcagct gctgaacgcc aaactgatca
      721 cacaacggaa gttcgataat ctgactaagg ctgaacgagg tggcctgtct gagttggata
      781 aagccggctt catcaaaagg cagcttgttg agacacgcca gatcaccaag cacgtg
//
