LOCUS       sequence-158458-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that expresses Cas9C in mammalian cells; co-expresses 
            turboGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pSMVP-Cas9C-P2A-turboGFP
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(661..717)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(736..756)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcgatgggg gtcttgaagg cgtgctggta ctccacgatg cccagctcgg tgttgctgtg
       61 cagctcctcc acgcggcgga aggcgaacat ggggcccccg ttctgcagga tgctggggtg
      121 gatggcgctc ttgaagtgca tgtggctgtc caccacgaag ctgtagtagc cgccgtcgcg
      181 caggctgaag gtgcgggcga agctgcccac cagcacgtta tcgcccatgg ggtgcaggtg
      241 ctccacggtg gcgttgctgc ggatgatctt gtcggtgaag atcacgctgt cctcggggaa
      301 gccggtgccc accaccttga agtcgccgat cacgcggccg gcctcgtagc ggtagctgaa
      361 gctcacgtgc agcacgccgc cgtcctcgta cttctcgatg cgggtgttgg tgtagccgcc
      421 gttgttgatg gcgtgcagga aggggttctc gtagccgctg gggtaggtgc cgaagtggta
      481 gaagccgtag cccatcacgt ggctcagcag gtaggggctg aaggtcaggg cgcctttggt
      541 gctcttcatc ttgttggtca tgcggccctg ctcgggggtg ccctctccgc cgcccaccag
      601 ctcgaactcc acgccgttca gggtgccggt gatgcggcac tcgatcttca tggcgggcat
      661 aggaccgggg ttttcttcca cgtcccctgc ttgctttaac agagagaagt tcgtggctcc
      721 ggatccagct cgagacacct tcctcttctt cttggggtca gccctgctgt ctccaccgag
      781 ctgagagagg tcgattcttg tttcatagag ccccgtaatt gactgatgaa tcagtgtggc
      841 gtccaggacc tcctttgtag aggtgtaccg ctttctgtct atggtggtgt cga
//
