LOCUS       sequence-158462-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9N in mammalian cells; expresses gRNA M4 for Mstn 
            cleavage..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pAAV-SMVP-Cas9N-U6-gRNA M4
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..55
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaagccgcta gcgctaccgg tcgccaccat ggccccaaag aagaagcgga aggtcggtat
       61 ccacggagtc ccagcagccg acaagaagta ctccattggg ctcgatatcg gcacaaacag
      121 cgtcggctgg gccgtcatta cggacgagta caaggtgccg agcaaaaaat tcaaagttct
      181 gggcaatacc gatcgccaca gcataaagaa gaacctcatt ggcgccctcc tgttcgactc
      241 cggggaaacg gccgaagcca cgcggctcaa aagaacagca cggcgcagat atacccgcag
      301 aaagaatcgg atctgctacc tgcaggagat ctttagtaat gagatggcta aggtggatga
      361 ctctttcttc cataggctgg aggagtcctt tttggtggag gaggataaaa agcacgagcg
      421 ccacccaatc tttggcaata tcgtggacga ggtggcgtac catgaaaagt acccaaccat
      481 atatcatctg aggaagaagc ttgtagacag tactgataag gctgacttgc ggttgatcta
      541 tctcgcgctg gcgcatatga tcaaatttcg gggacacttc ctcatcgagg gggacctgaa
      601 cccagacaac agcgatgtcg acaaactctt tatccaactg gttcagactt acaatcagct
      661 tttcgaagag aacccgatca acgcatccgg agttgacgcc aaagcaatcc tgagcgctag
      721 gctgtccaaa tcccggcggc tcgaaaacct catcgcacag ctccctgggg ag
//
