LOCUS       sequence-158472-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TM-MAGE strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Lennen RM, Nilsson Wallin AI, Pedersen M, Bonde M, Luo H, Herrgard 
            MJ, Sommer MO
  TITLE     Transient overexpression of DNA adenine methylase enables efficient 
            and mobile genome engineering with reduced off-target effects.
  JOURNAL   Nucleic Acids Res. 2016 Feb 29;44(4):e36. doi: 10.1093/nar/gkv1090. 
            Epub 2015 Oct 22.
  PUBMED    26496947
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-02-29"; volume: "44(4):e36. doi"; pages: " 
            10.1093/nar/gkv1090. Epub 2015 Oct 22"
COMMENT     Sequence Label: pMA7
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..793
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MGTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
ORIGIN
        1 attcaccatg ggtactgcac tcgcaacgct ggctgggaag ctggctgaac gtgtcggcat
       61 ggattctgtc gacccacagg aactgatcac cactcttcgc cagacggcat ttaaaggtga
      121 tgccagcgat gcgcagttca tcgcattact gatcgttgcc aaccagtacg gccttaatcc
      181 gtggacgaaa gaaatttacg cctttcctga taagcagaat ggcatcgttc cggtggtggg
      241 cgttgatggc tggtcccgca tcatcaatga aaaccagcag tttgatggca tggactttga
      301 gcaggacaat gaatcctgta catgccggat ttaccgcaag gaccgtaatc atccgatctg
      361 cgttaccgaa tggatggatg aatgccgccg cgaaccattc aaaactcgcg aaggcagaga
      421 aatcacgggg ccgtggcagt cgcatcccaa acggatgtta cgtcataaag ccatgattca
      481 gtgtgcccgt ctggccttcg gatttgctgg tatctatgac aaggatgaag ccgagcgcat
      541 tgtcgaaaat actgcataca ctgcagaacg tcagccggaa cgcgacatca ctccggttaa
      601 cgatgaaacc atgcaggaga ttaacactct gctgatcgcc ctggataaaa catgggatga
      661 cgacttattg ccgctctgtt cccagatatt tcgccgcgac attcgtgcat cgtcagaact
      721 gacacaggcc gaagcagtaa aagctcttgg attcctgaaa cagaaagccg cagagcagaa
      781 ggtggcagca tgaagtcggt gtggagtgaa acgatga
//
