LOCUS       sequence-158475-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein 
            LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford
            GE, Reddy TE, Gersbach CA
  TITLE     RNA-guided gene activation by CRISPR-Cas9-based transcription 
            factors.
  JOURNAL   Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
  PUBMED    23892895
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600."
COMMENT     Sequence Label: pcDNA-dCas9
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acctccacgt tcagctttcg ttaaattatc aaacttacgt tgagtgatta acttggcgtt
       61 tagaagttgt ctccaatagt ttttcatctt tttgactact tcttcacttg gaacgttatc
      121 cgatttacca cgatttttat cagaacgcgt taagacctta ttgtctattg aatcgtcttt
      181 aaggaaactt tgtggaacaa tggcatcgac atcataatca cttaaacgat taatatctaa
      241 ttcttggtcc acatacatgt ctcttccatt ttggagataa tagagataga gcttttcatt
      301 ttgcaattga gtattttcaa caggatgctc tttaagaatc tgacttccta attctttgat
      361 accttcttcg attcgtttca tacgctctcg cgaatttttc tggccctttt gagttgtctg
      421 attttcacgt gccatttcaa taacgatatt ttctggctta tgccgcccca ttactttgac
      481 caattcatca acaactttta cagtctgtaa aatacctttt ttaatagcag ggctaccagc
      541 taaatttgca atatgttcat gtaaactatc gccttgtcca gacacttgtg ctttttgaat
      601 gtcttcttta aatgtcaaac tatcatcatg gatcagctgc ataaaattgc gattggcaaa
      661 accatctgat ttcaaaaaat ctaatattgt tttgccagat tgcttatccc taataccatt
      721 aatcaatttt cgagacaaac gtccccaacc agtataacgg cgacgtttaa gctgtttcat
      781 caccttatca tcaaagaggt gagcatatgt tttaagtctt tcctcaatca tctccctatc
      841 ttcaaataag gtca
//
