LOCUS       sequence-158476-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector for expression of kinase active PAK2 with a CFP 
            fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Whalley HJ, Porter AP, Diamantopoulou Z, White GR, Castaneda-Saucedo
            E, Malliri A
  TITLE     Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal 
            Pak and promote mitotic spindle formation.
  JOURNAL   Nat Commun. 2015 Jun 16;6:7437. doi: 10.1038/ncomms8437.
  PUBMED    26078008
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-06-16"; volume: "6:7437. doi"; pages: " 
            10.1038/ncomms8437"
COMMENT     Sequence Label: pRetro-XT-PAK2-CFP(puro)-L107F
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agtccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
       61 catcaacaag tttgtacaaa aaagcaggct tgatgtctga taacggagaa ctggaagata
      121 agcctccagc acctcctgtg cgaatgagca gcaccatctt tagcactgga ggcaaagacc
      181 ctttgtcagc caatcacagt ttgaaacctt tgccctctgt tccagaagag aaaaagccca
      241 ggcataaaat catctccata ttctcaggca cagagaaagg aagtaaaaag aaagaaaagg
      301 aacggccaga aatttctcct ccatctgatt ttgagcacac catccatgtt ggctttgatg
      361 ctgttactgg agaattcact ggcatgccag aacagtgggc tcgattattt cagacctcca
      421 atatcaccaa actagagcaa aagaagaatc ctcaggctgt gctggatgtc ctaaagttct
      481 acgactccaa cacagtgaag cagaaatatc tgagctttac tcctcctgag aaagatggct
      541 ttccttctgg aacaccagca ctgaatgcca agggaacaga agcacccgca gtagtgacag
      601 aggaggagga tgatgatgaa gagactgctc ctcccgttat tgccccgcga ccggatcata
      661 cgaaatcaat ttacacacgg tctgtaattg accctgttcc tgcaccagtt ggtgattcac
      721 atgttgatgg tgctgccaag tctttagaca aacagaaa
//
