LOCUS       sequence-158486-        5432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA-tagged mouse FoxO1 in mammalian cells, PuroR selection 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Kong D, Gong L, Arnold E, Shanmugam S, Fort PE, Gardner TW, Abcouwer
            SF
  TITLE     Insulin-like growth factor 1 rescues R28 retinal neurons from 
            apoptotic death through ERK-mediated BimEL phosphorylation 
            independent of Akt.
  JOURNAL   Exp Eye Res. 2016 Aug 7;151:82-95. doi: 10.1016/j.exer.2016.08.002.
  PUBMED    27511131
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp Eye 
            Res."; date: "2016-08-7"; volume: "151:82-95. doi"; pages: " 
            10.1016/j.exer.2016.08.002"
COMMENT     Sequence Label: pSELECT-HA-mFOXO1
FEATURES             Location/Qualifiers
     source          1..5432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    9..57
                     /note="synthetic polyadenylation signal"
     misc_feature    68..159
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     108..127
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        196..407
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             420..688
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             739..765
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2817..2938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2828..2847)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2882..2901
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    3038..3432
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     promoter        complement(4055..4073)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4065..4111)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(4158..4360)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4187..4207)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4362..4665
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(4745..5333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4825..4844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
       61 cgtaactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
      121 ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgaaggatc tgcgatcgct
      181 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
      241 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
      301 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
      361 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag
      421 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt
      481 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg
      541 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta
      601 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt
      661 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacc tgagatcacc
      721 ggcgtgtcga ccgccatgta cccatacgat gttccggatt acgctgccga agcgccccag
      781 gtggtggaga ccgacccgga cttcgagccg ctgccccggc agcgctcctg tacctggccg
      841 ctgcccaggc cggagtttaa ccagtccaac tcgaccacct ccagtccggc gccgtcgggc
      901 ggcgcggccg ccaaccccga cgccgcggcg agcctggcct cggcgtccgc tgtcagcacc
      961 gactttatga gcaacctgag cctgctggag gagagtgagg acttcgcgcg ggcgccaggc
     1021 tgcgtggccg tggcggcggc ggctgcggcc agcaggggcc tgtgcgggga cttccagggc
     1081 cccgaggcgg gctgcgtgca cccagcgccg ccacagcccc caccgaccgg gccgctgtcg
     1141 cagcccccac ccgtgcctcc ctccgctgcc gccgccgcgg ggccactcgc gggacagccg
     1201 cgcaagacca gctcgtcgcg ccgcaacgcg tggggcaacc tgtcgtacgc cgacctcatc
     1261 accaaggcca tcgagagctc agccgagaag aggctcaccc tgtcgcagat ctacgagtgg
     1321 atggtgaaga gcgtgcccta cttcaaggat aagggcgaca gcaacagctc ggcgggctgg
     1381 aagaattcaa ttcgccacaa tctgtccctt cacagcaagt ttattcgagt gcagaatgaa
     1441 ggaactggaa agagttcttg gtggatgctc aatccagagg gaggcaagag cggaaaatca
     1501 ccccggagaa gagctgcgtc catggacaac aacagtaaat ttgctaagag ccgagggcgg
     1561 gctgctaaga aaaaagcatc tctccagtct gggcaagagg gtcctggaga cagccctggg
     1621 tctcagtttt ctaagtggcc tgcgagtcct gggtcccaca gcaacgatga ctttgataac
     1681 tggagtacat ttcgtcctcg aaccagctca aatgctagta ccatcagtgg gagactttct
     1741 cccatcatga cagagcagga tgacctggga gatggggacg tgcattccct ggtgtatcca
     1801 ccctctgctg ccaagatggc gtctacgctg cccagtctgt ctgaaatcag caatccagaa
     1861 aacatggaga accttctgga taatctcaac cttctctcgt ccccaacatc tttaactgtg
     1921 tccacccagt cctcgcctgg cagcatgatg cagcagacac catgctattc gtttgcaccg
     1981 ccaaacacca gtctaaattc acccagtcca aactactcaa agtacacata cggccaatcc
     2041 agcatgagcc ctttgcccca gatgcctatg cagacacttc aggacagcaa atcaagttac
     2101 ggaggattga accagtataa ctgtgcccca ggactcttga aagagttgtt gacttctgac
     2161 tctcctcccc acaatgacat tatgtcaccg gttgatcccg gagtggccca acccaacagt
     2221 cgggtcctgg gccaaaatgt aatgatgggc cctaattcgg tcatgccagc gtatggcagc
     2281 caggcatctc ataacaaaat gatgaacccc agctcccaca cccaccctgg acatgcacag
     2341 caaacggctt cggtcaacgg ccgtaccctg ccccatgtgg tgaacaccat gcctcacaca
     2401 tctgccatga accgcttgac ccccgtgaag acacctttac aagtgcctct gtcccacccc
     2461 atgcagatga gtgccctggg cagctactcc tcggtgagca gctgcaatgg ctatggtagg
     2521 atgggtgtcc tccaccagga gaagctccca agtgacttgg atggcatgtt tattgagcgc
     2581 ttggactgtg acatggagtc catcattcgg aatgacctca tggatggaga taccttggat
     2641 tttaactttg ataatgtgtt gcccaaccaa agcttcccac acagtgtcaa gactacaaca
     2701 cacagctggg tgtcaggcta agagttagtg agcaggctac atttaaaagt ccttcagatt
     2761 gtctgacagc aggaactgag gagcagtcca aagatgcgct agctggccag acatgataag
     2821 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2881 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2941 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     3001 aagcaagtaa aacctctaca aatgtggtat ggaattctaa aatacagcat agcaaaactt
     3061 taacctccaa atcaagcctc tacttgaatc cttttctgag ggatgaataa ggcataggca
     3121 tcaggggctg ttgccaatgt gcattagctg tttgcagcct caccttcttt catggagttt
     3181 aagatatagt gtattttccc aaggtttgaa ctagctcttc atttctttat gttttaaatg
     3241 cactgacctc ccacattccc tttttagtaa aatattcaga aataatttaa atacatcatt
     3301 gcaatgaaaa taaatgtttt ttattaggca gaatccagat gctcaaggcc cttcataata
     3361 tcccccagtt tagtagttgg acttagggaa caaaggaacc tttaatagaa attggacagc
     3421 aagaaagcga gcttctagct caggtttaag ctccaggctt ccttgtcatg caccaagttc
     3481 ttgggccttc tggaacctca acatcagctg tcacagtgaa tcccagtctt tcataaaaag
     3541 gcaggtttct gggagcagaa gtttccagaa aggcaggaac tccagccctt tcagcagctt
     3601 caactccagg cagaacaaca gcagatccca gaccctttcc ctggtggtca gggctcactc
     3661 caacagttgc cagaaaccaa gctggctctt ttggcctgtg tggtgccagc agaccttcca
     3721 tttgttgttg tgctgccagc ctgcttccag agagctcagc cattcttggt ccaatttcag
     3781 caaaaacagc accagcttca acagactcag gtgttgtcca aactgcaaca gcagctccat
     3841 catctgcaac ccaaactttt ccaatgtcca gtcccactct ggtgaggaag agttcttgca
     3901 gttctgtcac cctctcaatg tgcctgtcag ggtcaactgt gtgccttgtt gcagggtagt
     3961 ctgcaaaagc agcagccagt gttctcacag ctcttggaac atcatctctg gttgccagcc
     4021 tcactgtggg tttgtactca gtcatggtgg ccctcctata gtgagtcgta ttatactatg
     4081 ccgatatact atgccgatga ttaattgtca aaacagcgtg gatggcgtct ccagcttatc
     4141 tgacggttca ctaaacgagc tctgcttata tagacctccc accgtacacg cctaccgccc
     4201 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg atttactagt
     4261 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
     4321 cgctatccac gcccattgat gtactgccaa aaccgcatca tcatggtaat agcgatgact
     4381 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     4441 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     4501 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     4561 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     4621 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcctgc aggttaatta
     4681 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4741 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4801 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4861 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4921 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4981 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5041 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5101 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5161 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5221 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5281 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5341 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5401 tggtcatggc tagttaatta acatttaaat ca
//
