LOCUS       sequence-158488-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, 
            Hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith 
            A
  TITLE     Exit from pluripotency is gated by intracellular redistribution of 
            the bHLH transcription factor Tfe3.
  JOURNAL   Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
  PUBMED    23582324
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.03"; journalName: "Cell"; date: "2013-04-11- 
            11"; volume: "153"; issue: "2"; pages: "335-47"
COMMENT     Sequence Label: pPB-CAG-3xFLAG::Fnip1-pgk-hph
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    75..96
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(111..116,117..134,135..141)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    149..165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     154..176
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     173..189
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     173..189
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    220..244
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aaggcacagt cgaggctgat ccgctggcac gacaggtttc ccgactggaa agcgggcagt
       61 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      121 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      181 agctatgacc atgattacgc caagcttgtc gaccccatca ccactttgta caagaaagct
      241 gggtcttaaa ggagtatctg agcaacgtat ggagagtggg tgcttgctac cgcagccaga
      301 agaggaaggt cactggattc aatcccaaga accactccta gctccttgac atgaacacgc
      361 atctgtcccc tcaggtactc agacagcatt ttgctcttga agtataactc ctgtaaccgg
      421 tcttcaaggt gcattacaca aaagtttgga gataaattat gtttataaag ctgaagagtg
      481 gaatgaagca ggttggaaac aagactagag accaacacct cctttcccaa tttattatct
      541 gttactcgtc tctgactgct ggccacttga acagtccatt tatccatatc agctataata
      601 cagacagctt ctgctattgg ttcatctaac actggatgct gcacagcatg agacaaatct
      661 gataccaggc actgacggag cctctcatca tttccaattc cttgaagaac aaagtcagga
      721 acataagatg agcagtagcc acccagcaaa gatcttccaa agttggcaat gttgggctga
      781 acagcactca cttcaatcaa ttttgaccc
//
