LOCUS       sequence-158489-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Kras with a G12D Mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  TITLE     Mutant Ras Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBabe-Kras G12D
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             63..89
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             69..95
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             join(117..149,150..152,153..683)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     CDS             117..683
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     primer_bind     complement(695..715)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 atcctccctt tatccagccc tcactccttc tctaggcgcc ggccggatca atggcttcaa
       61 gttatccata tgatgtgccc gactatgcct cacttggagg accctctgga tccaccatga
      121 ctgaatataa acttgtggta gttggagctg acggcgtagg caagagtgcc ttgacgatac
      181 agctaattca gaatcatttt gtggacgaat atgatccaac aatagaggat tcctacagga
      241 agcaagtagt aattgatgga gaaacctgtc tcttggatat tctcgacaca gcaggtcaag
      301 aggagtacag tgcaatgagg gaccagtaca tgaggactgg ggagggcttt ctttgtgtat
      361 ttgccataaa taatactaaa tcatttgaag atattcacca ttatagagaa caaattaaaa
      421 gagttaagga ctctgaagat gtacctatgg tcctagtagg aaataaatgt gatttgcctt
      481 ccagaacagt agacacaaaa caggctcagg acttagcaag aagttatgga attcctttta
      541 ttgaaacatc agcaaagaca agacagggtg ttgatgatgc cttctataca ttagttcgag
      601 aaattcgaaa acataaagaa aagatgagca aagatggtaa aaagaagaaa aagaagtcaa
      661 agacaaagtg tgtaattatg taagtcgacc ctgtggaatg tgtgtcagtt agggtgtgga
      721 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      781 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
      841 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
      901 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
      961 gccgcctcgg cctct
//
