LOCUS       sequence-158496-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive Cre expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNXRVbt-HACre
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..128
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             132..158
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             165..191
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             210..230
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctaggatc tcgagctcaa gcttcgtacg
       61 ctgcaggtcg acggatcccc gggttaatta acgctagcat gtacccatac gatgttcctg
      121 actatgcggg ctacccgtat gacgtgccgg actatgcagg agcctatcct tatgacgttc
      181 cagattacgc tggttctaga tgtactccac caaagaagaa gagaaaggtt gcccctagca
      241 tggccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat gcaacgagtg
      301 atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt tctgagcata
      361 cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag ttgaataacc
      421 ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat cttcaggcgc
      481 gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg cttcatcgtc
      541 ggtccgggct gccacgacca agtgacagca atgctgtttc actggttatg cggcggatcc
      601 gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg
      661 atttcgacca ggttcgttca ctcatggaaa atagcgatcg ctgccaggat atacgtaatc
      721 tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt gccaggatca
      781 gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccata
//
