LOCUS       sequence-158497-       11874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human TRIM5 CypA fusion with H126Q mutation in CypA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Neagu MR, Ziegler P, Pertel T, Strambio-De-Castillia C, Grutter C, 
            Martinetti G, Mazzucchelli L, Grutter M, Manz MG, Luban J
  TITLE     Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins 
            engineered from human components.
  JOURNAL   J Clin Invest. 2009 Oct;119(10):3035-47. doi: 10.1172/JCI39354. Epub
            2009 Sep 8.
  PUBMED    19741300
REFERENCE   2  (bases 1 to 11874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI39354"; 
            journalName: "J Clin Invest"; date: "2009-10"; volume: "119"; issue:
            "10"; pages: "3035-47"
COMMENT     Sequence Label: pFUPI hT5Cyp-H126Q
FEATURES             Location/Qualifiers
     source          1..11874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2611..3821
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3658..3677
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3860..4459
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3860..3879)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4356..4376
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4468..5041
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4635..4652)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4862..4881
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      5055..5064
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6566..7154
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6619..6639)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7037..7048)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7157..7173)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7158..7174)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7679..7859
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7885..7902)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7891..8115
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8161..8589
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8248..8267)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8458..8479
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8598..8618)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8603..8932
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8783..8918
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8845..8864
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8980..9027
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9046..9420
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9550..9671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9587..9606)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9641..9660
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9720..9736)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9720..9736)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9733..9755)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9744..9760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9768..9774,9775..9792,9793..9798))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9813..9834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9951..9968)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10122..10710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10202..10221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10881..11672,11673..11741))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11504..11523
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11742..11846)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg
     2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc
     2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
     2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
     2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
     2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
     2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
     3001 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
     3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
     3121 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga
     3181 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     3241 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     3301 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     3361 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt
     3421 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     3481 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     3541 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     3601 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     3661 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     3721 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
     3781 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg gctgcaggtc
     3841 gactctagct agagccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3901 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     3961 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4021 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     4081 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4141 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4201 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4261 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4321 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4381 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4441 ccgcaagccc ggtgcctgag aattccgccc ctctccctcc ccccccccta acgttactgg
     4501 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     4561 gccgtctttt ggcaatgtga gggcccggaa acctggtcct gtcttcttga cgagcattcc
     4621 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     4681 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     4741 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     4801 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     4861 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     4921 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     4981 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     5041 accggtggtc tagagccacc atggcttctg gaatcctggt taatgtaaag gaggaggtga
     5101 cctgccccat ctgcctggaa ctcctgacac aacccctgag cctggactgc ggccacagct
     5161 tctgccaagc atgcctcact gcaaaccaca agaagtccat gctagacaaa ggagagagta
     5221 gctgccctgt gtgccggatc agttaccagc ctgagaacat acggcctaat cggcatgtag
     5281 ccaacatagt ggagaagctc agggaggtca agttgagccc agaggggcag aaagttgatc
     5341 attgtgcacg ccatggagag aaacttctac tcttctgtca ggaggacggg aaggtcattt
     5401 gctggctttg tgagcggtct caggagcacc gtggtcacca cacgttcctc acagaggagg
     5461 ttgcccggga gtaccaagtg aagctccagg cagctctgga gatgctgagg cagaagcagc
     5521 aggaagctga agagttagaa gctgacatca gagaagagaa agcttcctgg aagactcaaa
     5581 tacagtatga caaaaccaac gtcttggcag attttgagca actgagagac atcctggact
     5641 gggaggagag caatgagctg caaaacctgg agaaggagga ggaagacatt ctgaaaagcc
     5701 ttacgaactc tgaaactgag atggtgcagc agacccagtc cctgagagag ctcatctcag
     5761 atctggagca tcggctgcag gggtcagtga tggagctgct tcagggtgtg gatggcgtca
     5821 taaaaaggac ggagaacgtg accttgaaga agccagaaac ttttccaaaa aatcaaagga
     5881 gagtgtttcg agctcctgat ctgaaaggaa tgctagaagt gtttagagag ctgacagatg
     5941 tccgacgcta ctgggttgat gtgacagtgg ctccaaacaa catttcatgt gctgtcattt
     6001 ctgaagataa gagacaagtg agctctgtca accccaccgt gttcttcgac attgccgtcg
     6061 acggcgagcc cttgggccgc gtctcctttg agctgtttgc agacaaggtc ccaaagacag
     6121 cagaaaattt tcgtgctctg agcactggag agaaaggatt tggttataag ggttcctgct
     6181 ttcacagaat tattccaggg tttatgtgtc agggtggtga cttcacacgc cataatggca
     6241 ctggtggcaa gtccatctat ggggagaaat ttgaagatga gaacttcatc ctaaagcata
     6301 cgggtcctgg catcttgtcc atggcaaatg ctggacccaa cacaaatggt tcccagtttt
     6361 tcatctgcac tgccaagact gagtggttgg atggcaagca agtggtgttt ggcaaagtga
     6421 aagaaggcat gaatattgtg gaggccatgg agcgctttgg gtccaggaat ggcaagacca
     6481 gcaagaagat caccattgct gactgtggac aactcgaata aggatccgtt aacggcgcgc
     6541 cgaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     6601 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6661 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6721 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6781 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6841 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6901 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6961 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7021 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7081 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7141 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     7201 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     7261 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     7321 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7381 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     7441 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     7501 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     7561 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     7621 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     7681 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7741 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7801 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7861 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7921 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7981 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     8041 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     8101 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     8161 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8221 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8281 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8341 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8401 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8461 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8521 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8581 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     8641 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8701 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8761 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8821 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     8881 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     8941 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     9001 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     9061 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     9121 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     9181 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     9241 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     9301 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     9361 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     9421 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     9481 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     9541 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9601 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9661 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9721 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9781 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9841 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9901 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9961 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    10021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    10081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    10321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10441 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10561 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10741 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10801 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10861 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10921 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10981 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    11041 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    11101 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    11161 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11221 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11281 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11341 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11401 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11461 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11521 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11581 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11641 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11701 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11761 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11821 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
