LOCUS       sequence-158500-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains pBAD-CusR (response regulator) and 
            pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy 
            tracking experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH003
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..30)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(46..75)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgtttaaact caatgatgat gatgatgatg gtcgacggcg ctattcagat cctcttctga
       61 gatgagtttt tgttcgggcc caagcttctc actatttgta ttattaaact gctgcagcgt
      121 agttttcgtc gtttgctgct gcggtaccag aacctttgta tagttcatcc atgccatgtg
      181 taatcccagc agctgttaca aattccaaga gtaccatgtg gtctcttttt tcgttaggat
      241 cttttgacaa tttgctttga taggacagat aatggttatc gggtagcaaa actggtccat
      301 caccgatcgg ggtattttgt tggtaatgat cagcaagctg tactccacca tcctcaatat
      361 tgtgtctaat cttaaaattg gctttgattc cattcttctg cttgtcggcg gtaatgtaaa
      421 cgttgtgtga attatagtta tattcaagtt tatggcctag aatattacca tcctctttga
      481 aatcaatccc ctttaactct attcgattta caagtgtgtc accttcgaat ttaacttcag
      541 ccctagtttt ataattaccg tcatctttaa agaaaatagt acgttcttgg acgtaacctt
      601 ctggcattgc agatttaaag aagtcatgcc ttttcatgtg atctgggtat ctagcaaagc
      661 acatcaaccc ataacctaac gttgtcacca gggtaggcca gggaacaggc agcttgccgg
      721 ttgtacatat taacttcaaa gttaacttac cgtacgttgc atcaccctca ccttcgccgg
      781 agacactgaa cttatgaccg ttcacgtcgc catctagttc aactaaaatt gggacaactc
      841 cagtgaaa
//
