LOCUS       sequence-158504-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains pBAD-CusR (response regulator) and 
            pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH015
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..30)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(46..75)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(95..160)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cgtttaaact caatgatgat gatgatgatg gtcgacggcg ctattcagat cctcttctga
       61 gatgagtttt tgttcgggcc caagcttctc actacttgtc atcgtcatcc ttgtagtcga
      121 tgtcatgatc cttgtaatca ccgtcatggt ccttgtagtc tttgtacagt tcatccatac
      181 catgcgtgat gcccgctgcg gttacgaact ccagcagaac catatgatcg cgtttctcgt
      241 tcggatcttt agacagaacg ctttgcgtgc tcagatagtg attgtctggc agcagaacag
      301 gaccatcacc gattggagtg ttttgctggt agtgatcagc cagctgcacg ctgccatcct
      361 ccacgttgtg gcgaatttta aaattcgctt taatgccatt tttttgttta tcggcggtga
      421 tgtaaacatt gtggctgtta aaattgtatt ccagcttatg gcccaggata ttgccgtctt
      481 ctttaaagtc aatgcctttc agctcaatgc ggtttaccag ggtatcgcct tcaaatttca
      541 cttccgcacg cgttttgtac gtgccgtcat ccttaaagga aatcgtgcgt tcctgcacat
      601 agccttccgg catggcggac ttgaagaagt catgctgctt catatggtcc ggataacgag
      661 caaagcactg aacaccataa gtcagcgtcg ttaccagagt cggccaagga accggcagtt
      721 taccagtagt acagatgaac ttcagcgtca gtttaccatt agttgcgtca ccttcaccct
      781 cgccacgcac ggaaaactta tgaccgttga catcaccatc cagttccacc agaataggga
      841 cgacaccagt g
//
