LOCUS       sequence-158506-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains bicistronic pTet-Scaffold(3xFLAG)- ShineDelgarno-mCherry 
            and TetR genes. Used for Western blots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH011
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(139..204)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(457..474)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(536..554)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        632..687
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    633..651
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(779..798)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc
       61 gcatgaactc cttgatgatg gccatgttat cctcctcgcc cttgctcacc atgaattccc
      121 tccttaggat cctcactact tgtcatcgtc atccttgtag tcgatgtcat gatccttgta
      181 atcaccgtca tggtccttgt agtcgctgcc gctgccacta ccggatccca gggggccgta
      241 gcgggtttca tatttgctaa caatattctc gcagcggcct acttctttct ccagttcagc
      301 aatctcagta cgcagtgcgg tgttttcctt ctccagaaaa gctgcccgaa ttgtgataga
      361 tccaccgctg ccggacccag atccacgcct tctctttggc ggtaatgcag gtggaggctg
      421 gaagtacagg ttttcggtag tcgggatgtc gtagtcgtga tggtgatggt gatgcataga
      481 tctcttagtt tgcgttgggc cacacttcag gtaccgctag cgtgaagacg aaagggcctc
      541 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gtcgactcta
      601 gtggatctgc tagtcatatg gattttcact tttctctatc actgataggg agtggtaaaa
      661 taactctatc aacgatagag tgtcaacaaa aattaggaat taatgatgtc aagattagat
      721 aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta
      781 acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta
      841 aa
//
