LOCUS       sequence-158507-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains bicistronic pTet-Scaffold(3xFLAG)- ShineDelgarno-mCherry 
            and TetR genes. Used for Western blots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH011
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             286..315
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             331..348
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(404..421)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      574..620
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(662..679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
       61 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
      121 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
      181 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
      241 agctgtacaa gtaataataa ctcgagtaat gagaagcttg ggcccgaaca aaaactcatc
      301 tcagaagagg atctgaatag cgccgtcgac catcatcatc atcatcattg agtttaaacg
      361 gtctccagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat
      421 cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc
      481 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt
      541 ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa
      601 gactgggcct ttcgttttat ctgttgtttg tcggtgaact aatttctaga ctgcaggctt
      661 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      721 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      781 caaaaggcca gcaaaaggcc aggaaccgta aa
//
