LOCUS       sequence-158510-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dendra2 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Mohr MA, Argast P, Pantazis P
  TITLE     Labeling cellular structures in vivo using confined primed 
            conversion of photoconvertible fluorescent proteins.
  JOURNAL   Nat Protoc. 2016 Dec;11(12):2419-2431. doi: 10.1038/nprot.2016.134. 
            Epub 2016 Nov 3.
  PUBMED    27809312
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2016.134"; journalName: "Nat Protoc"; date: 
            "2016-12"; volume: "11"; issue: "12"; pages: "2419-2431"
COMMENT     Sequence Label: pGEX6P-1-Dendra2
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..41
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             51..740
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     primer_bind     complement(905..927)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc atgaacaccc
       61 cgggaattaa cctgatcaag gaggacatgc gcgtgaaggt gcacatggag ggcaacgtga
      121 acggccacgc cttcgtgatc gagggcgagg gcaagggcaa gccctacgag ggcacccaga
      181 ccgccaacct gaccgtgaag gagggcgccc ccctgccctt cagctacgac atcctgacca
      241 ccgccgtgca ctacggcaac cgggtgttca ccaagtaccc cgaggacatc cccgactact
      301 tcaagcagag cttccccgag ggctacagct gggagcgcac catgaccttc gaggacaagg
      361 gcatctgcac catccgcagc gacatcagcc tggagggcga ctgcttcttc cagaacgtgc
      421 gcttcaaggg caccaacttc ccccccaacg gccccgtgat gcagaagaag accctgaagt
      481 gggagcccag caccgagaag ctgcacgtgc gcgacggcct gctggtgggc aacatcaaca
      541 tggccctgct gctggagggc ggcggccact acctgtgcga cttcaagacc acctacaagg
      601 ccaagaaggt ggtgcagctg cccgacgccc acttcgtgga ccaccgcatc gagatcctgg
      661 gcaacgacag cgactacaac aaggtgaagc tgtacgagca cgccgtggcc cgctacagcc
      721 ccctgcccag ccaggtgtgg tccggcgaca gcggcgtgta caagtgaaag cttaattagc
      781 tgagcttgga ctcctgttga aagggcgaat tctgcagata tccatcacac tggcggccgc
      841 tcgagcggcc gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg
      901 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
      961 ggagcagaca agcccgtcag g
//
