LOCUS       sequence-158512-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for AAVS1 targeting (puromycin selection) and 
            constitutive mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pAAVS1-P-CAG-mCh
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    44..68
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(219..237)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     487..506
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     681..704
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 attcgcggcc gcggtaccgg cgcgccggat cctgcaggtt atcacaagtt tgtacaaaaa
       61 agcaggctcc accatggtga gcaagggcga ggaggacaac atggccatca tcaaggagtt
      121 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      181 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      241 cggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
      301 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
      361 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
      421 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
      481 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
      541 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
      601 ccactacgac gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      661 cgcctacaac gtcaacatca agctggacat cacctcccac aacgaggact acaccatcgt
      721 ggaacagtac gagcgcgccg agggccgcca ctcc
//
