LOCUS       sequence-158513-       10656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D
  TITLE     Reversible immortalization of human primary cells by 
            lentivector-mediated transfer of specific genes.
  JOURNAL   Mol Ther. 2000 Oct . 2(4):404-14.
  PUBMED    11020357
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2000 Oct . 2(4):404-14."
COMMENT     Sequence Label: pLOX-gfp-iresTK
FEATURES             Location/Qualifiers
     source          1..10656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..52)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     152..171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     385..404
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        694..712
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     694..711
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             737..917
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    937..970
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1126..2256)
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     misc_feature    2261..2722
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2420..2439)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2649..2666
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(2818..3528,3529..3531,3532..3534))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2818..3528,3529..3531,3532..3534))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     3209..3228
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3468..3489
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3531..3540
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3620..3644)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3641..3844)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3670..3690)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3845..4148
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(4427..4471)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4656..4889
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5382..5507
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5554..6187
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6355..6489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6379..6398)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6433..6452
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6914..6934)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7064..7129
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(8263..8592)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8277..8412
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8577..8597
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(8667..8684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8838..9426)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8918..8937)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9597..10388,10389..10457))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10220..10239
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10458..10562)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10630..10648
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
       61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
      181 caccattcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
      241 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
      301 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
      361 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
      421 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctggc tagcgatgac
      481 cctgctgatt ggttcgctga ccatttccgg gtgcgggacg gcgttaccag aaactcagaa
      541 ggttcgtcca accaaaccga ctctgacggc agtttacgag agagatgata gggtctgctt
      601 cagtaagcca gatgctacac aattaggctt gtacatattg tcgttagaac gcggctacaa
      661 ttaatacata accttatgta tcatacacat acgatttagg tgacactata gaatacacgg
      721 aattaattct agatgctgct agagattttc cacactgact aaaagggtct gagggatctc
      781 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
      841 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
      901 tctaaccaga gagacccagt acaagcaaaa agcagcataa cttcgtataa tgtatgctat
      961 acgaagttat tcccatggtg aattgatccc atcttgtctt cgttgggagt gaattagccc
     1021 ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat
     1081 tggtcttaaa ggtaccgtcg aggaattcgg taccccttcc gtgtttcagt tagcctcccc
     1141 catctcccgg gcaaacgtgc gcgccaggtc gcagatcgtc ggtatggagc cgggggtggt
     1201 gacgtgggtc tggaccatcc cggaggtaag ttgcagcagg gcgtcccggc agccggcggg
     1261 cgattggtcg taatccagga taaagacgtg catgggacgg aggcgtttgg ccaagacgtc
     1321 caaggcccag gcaaacacgt tgtacaggtc gccgttgggg gccagcaact cgggggcccg
     1381 aaacagggta aataacgtgt ccccgatatg gggtcgtggg cccgcgttgc tctggggctc
     1441 ggcaccctgg ggcggcacgg ccgtccccga aagctgtccc caatcctccc gccacgaccc
     1501 gccgccctgc agataccgca ccgtattggc aagcagcccg taaacgcggc gaatcgcggc
     1561 cagcatagcc aggtcaagcc gctcgccggg gcgctggcgt ttggccaggc ggtcgatgtg
     1621 tctgtcctcc ggaagggccc ccaacacgat gtttgtgccg ggcaaggtcg gcgggatgag
     1681 ggccacgaac gccagcacgg cctggggggt catgctgccc ataaggtatc gcgcggccgg
     1741 gtagcacagg agggcggcga tgggatggcg gtcgaagatg agggtgaggg ccgggggcgg
     1801 ggcatgtgag ctcccagcct cccccccgat atgaggagcc agaacggcgt cggtcacggc
     1861 ataaggcatg cccattgtta tctgggcgct tgtcattacc accgccgcgt ccccggccga
     1921 tatctcaccc tggtcgaggc ggtgttgtgt ggtgtagatg ttcgcgattg tctcggaagc
     1981 ccccagcacc tgccagtaag tcatcggctc gggtacgtag acgatatcgt cgcgcgaacc
     2041 cagggccacc agcagttgcg tggtggtggt tttccccatc ccgtgaggac cgtctatata
     2101 aacccgcagt agcgtgggca ttttctgctc caggcggact tccgtggctt cttgctgccg
     2161 gcgagggcgc aacgccgtac gtcggttgct atggccgcga gaacgcgcag cctggtcgaa
     2221 cgcagacgcg tgttgatggc aggggtacga agccatggta ttatcatcgt gtttttcaaa
     2281 ggaaaaccac gtccccgtgg ttcggggggc ctagacgttt tttaacctcg actaaacaca
     2341 tgtaaagcat gtgcaccgag gccccagatc agatcccata caatggggta ccttctgggc
     2401 atccttcagc cccttgttga atacgcttga ggagagccat ttgactcttt ccacaactat
     2461 ccaactcaca acgtggcact ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg
     2521 cttttggccg cagaggcacc tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc
     2581 tacagacgtt gtttgtcttc aagaagcttc cagaggaact gcttccttca cgacattcaa
     2641 cagaccttgc attcctttgg cgagagggga aagaccccta ggaatgctcg tcaagaagac
     2701 agggccaggt ttccgggccc tcacattgcc aaaagacggc aatatggtgg aaaataaccg
     2761 gaattaatgt cgactgcaga attaattcga agcttgagct cgagatctga gtccggactt
     2821 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
     2881 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt
     2941 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
     3001 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt
     3061 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
     3121 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
     3181 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
     3241 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
     3301 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
     3361 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
     3421 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
     3481 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc
     3541 gaccggtagc gctaggatcc tctagctaga gtcggtgtct tctatggagg tcaaaacagc
     3601 gtggatggcg tctccaggcg atctgacggt tcactaaacg agctctgctt atatagacct
     3661 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
     3721 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
     3781 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
     3841 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     3901 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     3961 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     4021 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
     4081 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     4141 atgtaacgcg gaactcccaa gcttatcgat accgtcgaga tccgttcact aatcgaatgg
     4201 atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc
     4261 tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca
     4321 ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga
     4381 ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt
     4441 tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta
     4501 aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt
     4561 gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg
     4621 tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc
     4681 tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca
     4741 gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca
     4801 ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg
     4861 ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca
     4921 ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt
     4981 tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg
     5041 gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct
     5101 tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta
     5161 tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt
     5221 tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc
     5281 ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga
     5341 attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct
     5401 ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg
     5461 cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc
     5521 cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa
     5581 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
     5641 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
     5701 cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat
     5761 atgcaggatc tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag
     5821 tccccagcgg aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga
     5881 tgcagctctc gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact
     5941 tctctctccg ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct
     6001 cctttattgg cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca
     6061 aaggtcagtg gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag
     6121 ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc ttctttggga gtgaattagc
     6181 ccttccaact actaagtttg tagtacatat ttaacaaata caatttcttt aaaatgaaaa
     6241 taattcagag gaatcacagg tttagagtaa atgaaaccac aggtaattgg cagtggtaat
     6301 agggtatggg gtgggaagtt tgggatgatt ttggttagct tgagttatcc agttgatcca
     6361 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     6421 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     6481 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     6541 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctctag
     6601 tcaaggcact atacatcaaa tattccttat taaccccttt acaaattaaa aagctaaagg
     6661 tacacaattt ttgagcatag ttattaatag cagacactct atgcctgtgt ggagtaagaa
     6721 aaaacagtat gttatgatta taactgttat gcctacttat aaaggttaca gaatattttt
     6781 ccataatttt cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag
     6841 caagagttct attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt
     6901 ccttggggtc ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag
     6961 tagcctcatc atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg
     7021 cattccacca ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa
     7081 ttagaatcag tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt
     7141 aaatctctgt aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa
     7201 agatccgggg cccactcata aatccagttg ccgccacggt agccaatcac cgtatcgtat
     7261 aaatcatcgt cggtacgttc ggcatcgctc atcacaatac gtgcctggac gtcgaggatt
     7321 tcgcgtgggt caatgccgcg ccagatccac atcagacggt taatcatgcg ataccagtga
     7381 gggatggttt taccatcaag ggccgactgc acaggcggtt gtgcgccgtg attaaagcgg
     7441 cggactagcg tcgaggtttc aggatgttta aagcggggtt tgaacagggt ttcgctcagg
     7501 tttgcctgtg tcatggatgc agcctccaga atacttactg gaaactattg taacccgcct
     7561 gaagttaaaa agaacaacgc ccggcagtgc caggcgttga aaagattagc gaccggagat
     7621 tggcgggacg aatacgacgc ccatatccca cggctgttca atccaggtat cttgcgggat
     7681 atcaacaaca tagtcatcaa ccagcggacg accagccggt tttgcgaaga tggtgacaaa
     7741 gtgcgctttt ggatacattt cacgaatcgc aaccgcagta ccaccggtat ccaccaggtc
     7801 atcaataacg atgaagcctt cgccatcgcc ttctgcgcgt ttcagcactt taagctcgcg
     7861 ctggttgtcg tgatcgtagc tggaaataca aacggtatcg acatgacgaa tacccagttc
     7921 acgcgccagt aacgcacccg gtacagaccg ccacggctta cggcaataat gcctttccat
     7981 tgttcagaag gcatcagtcg gcttgcgagt ttacgtgcat ggatctgcaa catgtcccag
     8041 gtgacgatgt atttttcgct catgtgaagt gtcccagcct gtttatctac ggcttaaaaa
     8101 gtgttcgagg ggaaaatagg ttgcgcgaga ttatagagat ctggcgcact aaaaaccagt
     8161 atttcacatg agtccgcgtc tttttacgca ctgcctctcc ctgacgcggg ataaagtggt
     8221 attctcaaac atatctcgca agcctgtctt gtgtccaagc tttttgcaaa agcctaggcc
     8281 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     8341 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     8401 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     8461 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     8521 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     8581 taactgacac acattccaca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8641 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8701 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8761 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8821 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8881 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8941 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9001 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9061 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9121 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9181 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9241 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9301 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9361 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9421 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9481 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9541 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9601 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9661 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9721 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9781 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9841 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9901 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9961 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10021 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10081 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10141 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10201 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10261 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10321 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10381 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10441 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10501 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10561 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
    10621 acctataaaa ataggcgtat cacgaggccc tttcgt
//
