LOCUS       sequence-158518-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes partial human gamma- and beta-globing cDNA for use as QPCR 
            standard as published in PMID: 14592835...
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Bhanu NV, Trice TA, Lee YT, Miller JL
  TITLE     A signaling mechanism for growth-related expression of fetal 
            hemoglobin.
  JOURNAL   Blood. 2004 Mar 1;103(5):1929-33. Epub 2003 Oct 30.
  PUBMED    14592835
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood."; 
            date: "2004-03-1"; volume: "103(5)"; pages: "1929-33. Epub 2003 Oct 
            30"
COMMENT     Sequence Label: BGGLO QPCR PLASMID
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..21
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10..32
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     29..45
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     29..45
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(569..588)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(570..588)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(595..612)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(595..611)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(604..626)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
       61 cttgaatgga ttgccaaaac ggtcaccagc acatttccca ggagcttgaa gttctcagga
      121 tccacatgca gcttgtcaca gtgcagttca ctcagctggg caaaggtgcc cttgaggtca
      181 tccaggtgct ttatggcatc tcccaaggaa gtcagcacct ccttgccatg tgccttgact
      241 ttggggttgc ccatgatggc agaggcagag gacaggttgc cactagtaac ggccgccagt
      301 gtgctggaat tcgcccttct catggcaaga aagtgctcgg tgcctttagt gatggcctgg
      361 ctcacctgga caacctcaag ggcacctttg ccacactgag tgagctgcac tgtgacaagc
      421 tgcacgtgga tcctgagaac ttcaggctcc tgggcaacgt gctggtctgt gtgctggccc
      481 atcactttgg caaagaatta agggcgaatt ctgcagatat ccatcacact ggcggccgct
      541 cgagcatgca tctagagggc ccaattcgcc ctatagtgag tcgtattaca attcactggc
      601 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      661 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      721 ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg
      781 gcgggtg
//
