LOCUS       sequence-158523-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  high fidelity SpCas9 mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     crispr/cas9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pX330-Flag-HFSpCas9-H840A
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acgactcaga aggggcaaaa aaacagtcga gagcggatga agagaataga agagggtatt
       61 aaagaactgg gcagccagat cttaaaggag catcctgtgg aaaataccca attgcagaac
      121 gagaaacttt acctctatta cctacaaaat ggaagggaca tgtatgttga tcaggaactg
      181 gacataaacc gtttatctga ttacgacgtc gatgcgatcg taccccaatc ctttttgaag
      241 gacgattcaa tcgacaataa agtgcttaca cgctcggata agaaccgagg gaaaagtgac
      301 aatgttccaa gcgaggaagt cgtaaagaaa atgaagaact attggcggca gctcctaaat
      361 gcgaaactga taacgcaaag aaagttcgat aacttaacta aagctgagag gggtggcttg
      421 tctgaacttg acaaggccgg atttattaaa cgtcagctcg tggaaacccg cgccatcaca
      481 aagcatgttg cgcagatact agattcccga atgaatacga aatacgacga gaacgataag
      541 ctgattcggg aagtcaaagt aatcacttta aagtcaaaat tggtgtcgga cttcagaaag
      601 gattttcaat tctataaagt tagggagata aataactacc accatgcgca cgacgcttat
      661 cttaatgccg tcgtagggac cgcactcatt aagaaatacc cgaagctaga aagtgagttt
      721 gtgtatggtg attacaaagt ttatgacgtc cgtaagatga tcgcgaaaag cgaacaggag
      781 ataggcaagg ctacagccaa atacttcttt tattctaaca ttatgaattt ctt
//
