LOCUS       sequence-158526-       12858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase based mini-gene to report splicing at exon 25 in 
            Drosophila parayltic .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12858)
  AUTHORS   Lin WH, He M, Baines RA
  TITLE     Seizure suppression through manipulating splicing of a voltage-gated
            sodium channel.
  JOURNAL   Brain. 2015 Apr;138(Pt 4):891-901. doi: 10.1093/brain/awv012. Epub 
            2015 Feb 12.
  PUBMED    25681415
REFERENCE   2  (bases 1 to 12858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Brain."; 
            date: "2015-04"; volume: "138(Pt 4):891-901. doi"; pages: " 
            10.1093/brain/awv012. Epub 2015 Feb 12"
COMMENT     Sequence Label: exon L-firefly
FEATURES             Location/Qualifiers
     source          1..12858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             8476..10128
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(8542..8560)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             10164..10205
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             10215..10232
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10255..10272)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10305..10439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10329..10348)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10383..10402
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(11040..11628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11120..11139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11799..12590,12591..12659))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12422..12441
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12660..12764)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12832..12850
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 catggcatta gaagatgtac atctgccaca aagacccata ctgcaggata ttttatacta
     2641 tatggacaga atatttacgg ttatattctt cttggaaatg ttaatcaagt ggttggcgct
     2701 cggcttcaaa gtgtacttca ccaacgcgtg gtgttggctc gatttcgtga ttgtcatggt
     2761 aagtccaccg gttacccaag tagatcgctc cccaatcagc tttcggacct caatattttg
     2821 tttgccgaac gtatcgaaac cgtttaaaac ttaaccaacc acaagaccct ggaccgaaat
     2881 gaattgagtt acttgaagac gatttaagac ttttcagtta agcctttagt ctgtatgcct
     2941 tattcccgcc ccgaaatcgc tgtgtactgc ttagggcagt atgacagatg tcttgagaaa
     3001 cccagctcgt cccgagtttg tatctaaagt gtatattgag aaccgttgta tgatataaag
     3061 ccaaagaaca agtaactagt acactgtaac gagtaacgag taaacgtaaa gaatttgttt
     3121 gatattacca taggttagca tagttattca cattagctta gtacgataag tgccctctat
     3181 acatatactc gtatatagat catccctata gacatagctt atccaacttt gagtttcgtt
     3241 ttgagttacc cgccaatcta aatcgcccat ctagctgtcg ctaattaatt tggccgcggt
     3301 ctggtccggg gccgatgatg tgcccgcctt tcgctcgatg cgaactctgc gcgcctagcg
     3361 acctctgcgg gcagtttcgc gctgggaggg catgaaagta agtagcgtcc accacagcct
     3421 ctctatctct ctatctctct ctctctctct cgctgtctct gtgtgtctct cttgctcttt
     3481 ctcctcctta aaagttctaa gctaagctca gactaaattc ctatatatgt ttctcgctgt
     3541 gtaactttcc ctcattcgca ttcactgttg tctctatgtg tgtgcgggtg ggtgtgtgtg
     3601 cgtgcgtgta tgtgtgtgtg tgctaagtat acaactaata atactcaata gctatataca
     3661 ttcgttaaat gttgttgctt cactaacaaa ctgagttcct tacttgcggt ctatctaaat
     3721 ggcgagctct acaatcttca atcacatttg tatctttaaa aaaactgtta tacttcaacc
     3781 gctacgatct tcagctgggt tgtacacggt gcagcgacta tttgaataat tgtaaagcgc
     3841 cgtttacaac tatcgcaaaa cggttgaaag cttttaggat cgaacattga ccgcaacttc
     3901 gttggcaaag cgctttaatt cccgcctccg aaattgttat acacacacaa aatacatgtt
     3961 cgagcaatgt tattaatcac cgtcagtttt caatattgtg ttctgagcat acatatatcg
     4021 atatttaagc catctatgtg cgtgtgtatc tatgggtata cgtggttttc tttcgctcaa
     4081 tatgtgtgtg ctaaacgcta caatccgtcc aagccgaatc gctcacattg cgcccagggc
     4141 agcgccatgc taagtgttat accggccagt tctgtacatt ttctgctgcc tgttaatgtt
     4201 gatgtaattt actcctacat atgtttgtcc ctgccaaacc ccaaatatat atacatatat
     4261 atattgatac cattgtccct atatatacaa atattgttgc gtgtaaatgt aaagtaaaag
     4321 taaaatctac aagccaaccg caatatcttc aagaagcttt gtaggcattg tattccattg
     4381 ctgccgtcgc gcgcttcagc ttagctgcct acattgtata acagtagcgt aatcatacgg
     4441 ttatagttta aacaagtaat ctaggaccat tgatacacat acatacatag gtcagtcata
     4501 gcttttaaac tgtactatat ataatattcc tacgtatata atgaaattag aaataggaac
     4561 catacatgca acacgattgg tcagctaatg taactgttat acgagtacaa gcgtataata
     4621 acttgtgtat aagtagtttg gttcaataac tagccatagc ttagtcattg gtagcaatac
     4681 ctcccccaga acctcgaaac cccatgtaca ctcccagttg ccaagtccga acccctagat
     4741 cgttttggtt gtcttagaca gttagtccta gttaatccca ttaccaaagc cacgtccaca
     4801 tgaacacaac gaatttgaac cggaactgga ccccttccct cgccacggcc tccaacttag
     4861 cggcacaaaa ctatatctat ccgtataggt gtatgtatgt acataccaaa tgtatatccc
     4921 cataattgtt aaaaagcgaa ccacgcaaaa gggccacaac tgcactgaat tgtacaaaca
     4981 agaagtataa caaacaaatg taaccaaata taccaaaaac ttcacgacgc tcagtcgtta
     5041 gtgtatagat atcgaaatcg tttactcgta tatgtatata tactgtatat tcaaccacaa
     5101 tcagtacaat cagaacaaca accagatgtc gagtattcga taaatcgctc atacgcagtg
     5161 gtggcagtgt tttacttaca acaaacgacg ctccaatcgc gtcccgtctc acaccaacag
     5221 atatcgcttg gtctgggcta acttaactga gctcttttcc atctggggct aaactcaccc
     5281 caagattaac ttcagtccag tgcaaactgg gatccacaat acccaatcga gtccggcagc
     5341 aaagtttgag caaaaatgtg tcaacataga gacagatcaa gacaggaaaa ccaaaagccg
     5401 aaagccattt ttcaatgaga acctagtgat gaggtcgcac gactgcacaa tggactcatt
     5461 gaggtccctg catacgccgc gaccaacacc aaattcgtca taaagtaaag tttgtatata
     5521 ttagaataat ctatcgaatc atggaacatc cattctcgat ctctcgtcaa tcgtttcgtt
     5581 tcgtttcgtt tcgtccatgg cgtatgcatg ccgaattcgt ccattgtgga gtgtggtgcg
     5641 gctggtggtg caaacaatcg taggaaacaa tcgatttaga cagacctagc ctagttccca
     5701 aagtcataac tctctctgta actctctgga tgtgccgtct cccccccaaa agatcctttg
     5761 ctatcaagaa aggacctacc cgatttcagt aattcgtact cgtactcgta tcgaacaaca
     5821 cacacacaca ctcagctctc gtctctctat gtatgtctca cccagagcag ttatcgtata
     5881 cctactacta ctatataccc ttggattgaa ccgaaactac ttccaataca catcgaacca
     5941 atccgaaaaa caatcgttaa cgaaccgaac ccacagtaat ctacatatac gtactttata
     6001 tgcatatgtg ttaacctgtt ttgtgtgtat gtgtctacgt ctataagtgt taccatctat
     6061 gtatttgcca gaatatatac atataatcgt acaagctata ttatgtaaca actatcgtac
     6121 tacactaggc tattgtttaa gaacatgttt tgtctaagtc agaagagcgc attggtaata
     6181 tgcaagtaag ttctccgaac ccatagggcc cctgccacgc cccgttctgc cccttttttt
     6241 gcctttgacc cctttttgtc gaacagcagc tgcagctgca tttgaaaaac aacaacggcg
     6301 cggattaatc tctttaaagc ttcttttcgt tagacagaca gaaactaaaa cttaaaactt
     6361 aaaaaaaaaa aacaaaaaaa aaaacccaaa acccgatacc gaaaaaaccg aacaaagtac
     6421 cgaaaaatga aagataaatc acttttgctt tatttacaaa gaatctctaa gtatgcaact
     6481 aaatatgtta aacttggcac tattcgaact atatcgaaac taggtatcgc ttatcaactt
     6541 cgttgcttca cttgttggag ctggtggtat tcaagccttc aagactatgc gaacgttaag
     6601 agcactgaga ccactacgtg ccatgtcccg tatgcagggc atgagggtac gtactttcca
     6661 tacatactcg taacaaccca ataccaaacc aatatcaaat caaatcaaat cgaaaccaat
     6721 taccacaaca aaaaatactt accaacaaac aaacaaacaa tttcatcaat cagtatcagt
     6781 atcagtatca gtagcatatc gtatatcgta tctcaatcca gcaaatagcg ccaaatctca
     6841 tcataactaa tactggcgac tcctttacgc cgatacaacc tctctactcg aactcgttaa
     6901 caccagccat tcatttcaaa ctaacagcaa ccgatccgaa ttgtgtagct atagaaattg
     6961 aaacgtgcat agctttacag ttaaccattt ggattgatgg gctcataacc atgataatct
     7021 gcatcagtga agcagttgaa ttcatttgtt gcatccgtaa tttgtaaaac ttatgaaatt
     7081 atcaacataa atcgtaattg attgactgat tagatgctaa ttacccaaca acttactcaa
     7141 atctctctct ctcatcgatc agccgaacac aaaaaccaca ctccaagtac tagagttaat
     7201 cttagctatc gttaatcgtt attcttacat acttacttat gcatattgtt ataaataatt
     7261 agacaactgt tttgccttgc cttctctcac acacaagttg cgtcttgctc gatctctttt
     7321 tcgctctagc tagctcgctc tgtctatctc tctctgtttc tctctctctc tctctctctc
     7381 tctctcttat tttgacctac tatccacatc ctttattgat tgtccactcg gctcacactt
     7441 agaatataat tggctcatca gaacaaagaa attcgcttaa acagcttaac caattgacga
     7501 ctatcggtcg agtcctatgc ttgccaagta ctgtagaacc gatcgaacat tataattaga
     7561 attatcatcg ccttatgtac cagccacaca acttttgatt ctactcgtac tttgaccatc
     7621 tcatctagtc acgtacttag tctctagttt aagttaactc ttagctaatt ttgtcgcata
     7681 tacttaataa cttcaagttg aacatgtgct tgtttgttcg caaatgaacc gttatgctgt
     7741 tgcccgttgt gcctaactgt tatatatgta tgtagtattg taaagtaaac ggctgccttg
     7801 gcttggctca gtgttgagaa tcgcacgtcg cctgcaagta gtgttagtca gcacaccgag
     7861 aaagtctcca ttgcactcac acagtcgcac gctttcatct catacacaat aaaaaaattt
     7921 aaaaaaaaaa acaattattc atctatacag caatacactc attcacaaaa caaccatgta
     7981 cacgcaataa cacacatctg tacatcaaca tcattattct taagtctgat actaacttga
     8041 actagtgtat actgataatt atatatctat atattcccaa ctgactaaat aactctctgc
     8101 atttattgta ttatattcta tcccctctcc ccgccctcgc ctcgcctcgc cttgcctcgc
     8161 atcacccata cacccatgta tcccaactac tacaacaacc aataaaacaa ccaaccaacc
     8221 aatccatcga taactcaact cccaatcatc atcgacgttg ccaacgacga cccaatcgat
     8281 taacgatcga atgattgatc aatcaatcca cgaattcaac aaaacaaaaa accaaaccaa
     8341 attcaggtcg tcgttaatgc gctggtacaa gctataccgt ccatcttcaa tgtgctattg
     8401 gtgtgtctaa tattttggct aatttttgcc ataatgggtg tacagctttt tgctggaaaa
     8461 tattttaagt ctagaatgga agacgccaaa aacataaaga aaggcccggc gccattctat
     8521 ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     8581 gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag
     8641 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     8701 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     8761 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     8821 aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa
     8881 attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa
     8941 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     9001 tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc
     9061 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc
     9121 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     9181 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     9241 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg
     9301 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc
     9361 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt
     9421 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt
     9481 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     9541 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     9601 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct
     9661 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     9721 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt
     9781 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa
     9841 tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat
     9901 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     9961 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
    10021 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
    10081 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaagc ggccgctcga
    10141 gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
    10201 ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga
    10261 ctgtgccttc taaggcctga gctcgctgat cagcctcgat cgaggatcca gacatgataa
    10321 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
    10381 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
    10441 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
    10501 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtcg accgatgccc
    10561 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
    10621 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
    10681 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
    10741 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
    10801 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
    10861 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
    10921 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
    10981 ggacagcttc aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    11041 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    11101 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    11161 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    11221 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    11281 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    11341 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    11401 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    11461 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
    11521 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    11581 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    11641 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    11701 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    11761 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    11821 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    11881 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    11941 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    12001 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    12061 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
    12121 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    12181 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    12241 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    12301 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    12361 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
    12421 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    12481 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    12541 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    12601 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    12661 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    12721 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    12781 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    12841 atcacgaggc cctttcgt
//
