LOCUS       sequence-158529-       10253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression copGFP and Puromycine, with an S/MAR element for episomal
            maintaince.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Jin C, Fotaki G, Ramachandran M, Nilsson B, Essand M, Yu D
  TITLE     Safe engineering of CAR T cells for adoptive cell therapy of cancer 
            using long-term episomal gene transfer.
  JOURNAL   EMBO Mol Med. 2016 May 6. pii: e201505869. doi: 
            10.15252/emmm.201505869.
  PUBMED    27189167
REFERENCE   2  (bases 1 to 10253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Mol 
            Med. 2016 May 6. pii: e201505869. doi: 10.15252/emmm.201505869."
COMMENT     Sequence Label: pBMN(CMV-copGFP-Puro-SMAR)
FEATURES             Location/Qualifiers
     source          1..10253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      254..389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    429..550
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(440..459)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     494..513
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             622..802
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(845..1059)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(905..924)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             1277..1288
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1686..1706
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3963..4562)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4046..4066)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4543..4562
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(4575..4640)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             complement(4662..4718)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4734..4787)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(4869..5531)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     regulatory      5552..5561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5612..5636)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5633..5836)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5662..5682)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    5926..6042
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6309..6353)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6538..6771
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7264..7389
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7436..7616
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7617..7843)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(7858..7875)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7858..7874)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7867..7889)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8083..8102)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8202..8224
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8262..8280)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8348..8452
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8453..8521,8522..9313)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8671..8690)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9484..10072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10226..10243
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttggtct
      241 ctgcaaaagt ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
      301 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
      361 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggat agctagagcc
      421 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
      481 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
      541 taaacaagtt cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa
      601 taagatgaca tgaactacta ctgctagaga ttttccacac tgactgaaag ggtctgaggg
      661 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
      721 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
      781 tctggtctaa ccagagagac ccagtagaag caaaaagcag aatcgaagaa ttcagaggat
      841 ccaagtggtc tcatacagaa cttataagat tcccaaatcc aaagacattt cacgtttatg
      901 gtgatttccc agaacacata gcgacatgca aatattgcag ggcgccactc ccctgtccct
      961 cacagccatc ttcctgccag ggcgcacgcg cgctgggtgt tcccgcctag tgacactggg
     1021 cccgcgattc cttggagcgg gttgatgacg tcagcgttcc aattcttgac atcgttggga
     1081 gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc
     1141 cttgtaagtc attggtctta aaggtaccag gcggggaggc ggcccaaagg gagatccgac
     1201 tcgtctgagg gcgaaggcgg agacgcggaa gaggccgcag agccggcagc aggccgcggg
     1261 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
     1321 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca
     1381 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
     1441 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
     1501 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
     1561 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
     1621 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
     1681 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
     1741 tgtaatccag aggttgatct agcagcaagg tcgccacgca caagatcaat attaacaatc
     1801 agtcatctct ctttagcaat aaaaaggtga aaaattacat tttaaaaatg acaccataga
     1861 cgatgtatga aaataatcta cttggaaata aatctaggca aagaagtgca agactgttac
     1921 ccagaaaact tacaaattgt aaatgagagg ttagtgaaga tttaaatgaa tgaagatcta
     1981 aataaactta taaattgtga gagaaattaa tgaatgtcta agttaatgca gaaacggaga
     2041 gacatactat attcatgaac taaaagactt aatattgtga aggtatactt tctttccaca
     2101 taaatttgta gtcaatatgt tcaccccaaa aaagctgttt gttaacttgc caacctcatt
     2161 ctaaaatgta tatagaagcc caaaagacaa taacaaaaat attcttgtag aacaaaatgg
     2221 gaaagaatgt tccactaaat atcaagattt agagcaaagc atgagatgtg tggggataga
     2281 cagtgaggct gataaaatag agtagagctc agaaacagac ccattgatat atgtaagtga
     2341 cctatgaaaa aaatatggca ttttacaatg ggaaaatgat gatctttttc ttttttagaa
     2401 aaacagggaa atatatttat atgtaaaaaa taaaagggaa cccatatgtc ataccataca
     2461 cacaaaaaaa ttccagtgaa ttataagtct aaatggagaa ggcaaaactt taaatctttt
     2521 agaaaataat atagaagcat gccatcatga cttcagtgta gagaaaaatt tcttatgact
     2581 caaagtccta accacaaaga aaagattgtt aattagattg catgaatatt aagacttatt
     2641 tttaaaatta aaaaaccatt aagaaaagtc aggccataga atgacagaaa atatttgcaa
     2701 caccccagta aagagaattg taatatgcag attataaaaa gaagtcttac aaatcagtaa
     2761 aaaataaaac tagacaaaaa tttgaacaga tgaaagagaa actctaaata atcattacac
     2821 atgagaaact caatctcaga aatcagagaa ctatcattgc atatacacta aattagagaa
     2881 atattaaaag gctaagtaac atctgtggca atattgatgg tatataacct tgatatgatg
     2941 tgatgagaac agtactttac cccatgggct tcctccccaa acccttaccc cagtataaat
     3001 catgacaaat atactttaaa aaccattacc ctatatctaa ccagtactcc tcaaaactgt
     3061 caaggtcatc aaaaataaga aaagtctgag gaactgtcaa aactaagagg aacccaagga
     3121 gacatgagaa ttatatgtaa tgtggcattc tgaatgagat cccagaacag aaaaagaaca
     3181 gtagctaaaa aactaatgaa atataaataa agtttgaact ttagtttttt ttaaaaaaga
     3241 gtagcattaa cacggcaaag ccattttcat atttttcttg aacattaagt acaagtctat
     3301 aattaaaaat tttttaaatg tagtctggaa cattgccaga aacagaagta caacagctat
     3361 ctgtgctgtc gcctaactat ccatagctga ttggtctaaa atgagataca tcaacgctcc
     3421 tccatgtttt ttgttttctt tttaaatgaa aaactttatt ttttaagagg agtttcaggt
     3481 tcatagcaaa attgagagga aggtacattc aagctgagga agttttcctc tattcctagt
     3541 ttactgagag attgcatcat gaatgggtgt taaattttgt caaatgcttt ttctgtgtct
     3601 atcaatatga ccatgtgatt ttcttcttta acctgttgat gggacaaatt acgttaattg
     3661 attttcaaac gttgaaccac ccttacatat ctggaataaa ttctacttgg ttgtggtgta
     3721 tattttttga tacattcttg gattcttttt gctaatattt tgttgaaaat gtttgtatct
     3781 ttgttcatga gagatattgg tctgttgttt tcttttcttg taatgtcatt ttctagttcc
     3841 ggtattaagg taatgctggc ctagttgaat gatttaggaa gtattccctc tgcttctgtc
     3901 ttctgaaaga gattgtagaa agttgataca attttttttt ctttaaatat ttgataggat
     3961 catcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
     4021 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
     4081 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
     4141 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg
     4201 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
     4261 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
     4321 ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg
     4381 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg
     4441 cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg
     4501 cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc
     4561 atcccggggt cgactgggcc tgggttggac tccacgtctc ccgccaactt gagaaggtca
     4621 aaattcaaag tctgtttcac cggtccggag ccattaatta agggaccggg gttttcttcc
     4681 acgtctcctg cttgctttaa cagagagaag ttcgtggcac ctgatccgct agcgggccca
     4741 ggattctcct cgacgtcacc gcatgttagc agacttcctc tgccctcccc ggagccctcg
     4801 cgagatccgg tggagccggg tccggcggtg ccgtccacgg cagaattgga cgactgagcg
     4861 cgggatctgg cgaaggcgat gggggtcttg aaggcgtgct ggtactccac gatgcccagc
     4921 tcggtgttgc tgtgcagctc ctccacgcgg cggaaggcga acatggggcc cccgttctgc
     4981 aggatgctgg ggtggatggc gctcttgaag tgcatgtggc tgtccaccac gaagctgtag
     5041 tagccgccgt cgcgcaggct gaaggtgcgg gcgaagctgc ccaccagcac gttatcgccc
     5101 atggggtgca ggtgctccac ggtggcgttg ctgcggatga tcttgtcggt gaagatcacg
     5161 ctgtcctcgg ggaagccggt gcccaccacc ttgaagtcgc cgatcacgcg gccggcctcg
     5221 tagcggtagc tgaagctcac gtgcagcacg ccgccgtcct cgtacttctc gatgcgggtg
     5281 ttggtgtagc cgccgttgtt gatggcgtgc aggaaggggt tctcgtagcc gctggggtag
     5341 gtgccgaagt ggtagaagcc gtagcccatc acgtggctca gcaggtaggg gctgaaggtc
     5401 agggcgcctt tggtgctctt catcttgttg gtcatgcggc cctgcttggg ggtgccctct
     5461 ccgccgccca ccagctcgaa ctccacgccg ttcagggtgc cggtgatgcg gcactcgatc
     5521 tccatggcgg gcaggccgct ctcgtcgctc tccatggtgg cgtctagaat cttctatgga
     5581 ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc
     5641 ttatataaac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     5701 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     5761 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     5821 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     5881 gaaagtccca taaggtcatg tactgggcat aatactagta tcgataaaat tttgaatttt
     5941 gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt
     6001 tcccctgcac tgtacccccc aatcccccct tttcttttaa aagttaaccg ataccgtcga
     6061 gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt
     6121 ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa
     6181 tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct taaacctacc
     6241 aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac
     6301 caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc
     6361 tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg
     6421 tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta
     6481 gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg
     6541 agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg
     6601 ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc
     6661 tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct
     6721 gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc tattcccact
     6781 gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac tcctaatggt
     6841 tcaattttta ctactttata tttatataat tcacttctcc aattgtccct catatctcct
     6901 cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc
     6961 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
     7021 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
     7081 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt
     7141 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
     7201 ctttccccct ggccttaacc gaattttttc ccatcccgat ctaattctcc cccgcttaat
     7261 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
     7321 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaaacc gagtcctgcg
     7381 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
     7441 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     7501 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     7561 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccgttt
     7621 atgtatcgag ctaggcactt aaatacaata tctctgcaat gcggcaattc agtggttcgt
     7681 ccaatccatg tcagacccgt ctgttgcctt cctaataagg cacgatcgta ccaccttact
     7741 tccaccaatc ggcatgcacg gtgctttttc tctccttgta aggcatgttg ctaactcatc
     7801 gttaccatgt tgcaagacta caagagtatt gcataagact acacgcgtca gcttggcact
     7861 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     7921 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     7981 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     8041 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     8101 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     8161 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     8221 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     8281 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     8341 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8401 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8461 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8521 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8581 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8641 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8701 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8761 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8821 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8881 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8941 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9001 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9061 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9121 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9181 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9241 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9301 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9361 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9421 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9481 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9541 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9601 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9661 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9721 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9781 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9841 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9901 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9961 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    10021 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    10081 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    10141 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    10201 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag
//
