LOCUS       sequence-158536-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for Citrine fluorescent protein with a nuclear 
            localization signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Ogasawara Y, Ishizaki K, Kohchi T, Kodama Y
  TITLE     Cold-induced organelle relocation in the liverwort Marchantia 
            polymorpha L.
  JOURNAL   Plant Cell Environ. 2013 Feb 20. doi: 10.1111/pce.12085.
  PUBMED    23421791
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Environ. 2013 Feb 20. doi: 10.1111/pce.12085."
COMMENT     Sequence Label: pDONR207-Citrine-NLS
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    163..262
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(267..287)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(291..542)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(333..354)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     682..701
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     941..962
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
       61 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
      121 atcagagatt ttgagacacg ggccagagct gcagctggat ggcaaataat gattttattt
      181 tgactgatag tgacctgttc gttgcaacaa attgataagc aatgctttct tataatgcca
      241 actttgtaca agaaagctgg gtctcacacc ttgcgcttct tcttaggagg cttgtacagc
      301 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
      361 cgcttctcgt tggggtcttt gctcagggcg gactggtagc tcaggtagtg gttgtcgggc
      421 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
      481 ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg
      541 tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg
      601 ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc
      661 tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc
      721 tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg
      781 gggtagcggg cgaagcacat caggccgtag ccgaaggtgg tcacgagggt gggccagggc
      841 acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg
      901 ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc
      961 aggatg
//
