LOCUS       sequence-158539-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mApple-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..64
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     15..35
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..149
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      165..174
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             171..881
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     584..603
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tccgccccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
       61 agctggttta gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca
      121 agaagttcga gtccatctcc aaggaggagg gggatccacc ggtcgccacc atggtgagca
      181 agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
      241 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      301 aggcctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      361 acatcctgtc ccctcagttc atgtacggct ccaaggtcta cattaagcac ccagccgaca
      421 tccccgacta cttcaagctg tccttccccg agggcttcag gtgggagcgc gtgatgaact
      481 tcgaggacgg cggcattatt cacgttaacc aggactcctc cctgcaggac ggcgtgttca
      541 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
      601 agaccatggg ctgggaggcc tccgaggagc ggatgtaccc cgaggacggc gccctgaaga
      661 gcgagatcaa gaagaggctg aagctgaagg acggcggcca ctacgccgcc gaggtcaaga
      721 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt
      781 tggacatcgt gtcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
      841 gccgccactc caccggcggc atggacgagc tgtacaagta agcggccgcg actctagatc
      901 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      961 cccctgaacc tgaaacataa aatgaatgca att
//
