LOCUS       sequence-158541-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize
            using Asc1 and Not1 for mouse embryo injection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Mohr MA, Argast P, Pantazis P
  TITLE     Labeling cellular structures in vivo using confined primed 
            conversion of photoconvertible fluorescent proteins.
  JOURNAL   Nat Protoc. 2016 Dec;11(12):2419-2431. doi: 10.1038/nprot.2016.134. 
            Epub 2016 Nov 3.
  PUBMED    27809312
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2016.134"; journalName: "Nat Protoc"; date: 
            "2016-12"; volume: "11"; issue: "12"; pages: "2419-2431"
COMMENT     Sequence Label: p55-H2B-Dendra2
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             78..455
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 catgttcatg ccttcttctt tttcctacag atccttaatt aataatacga ctcactatag
       61 ggggtcgacc cgccaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct
      121 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
      181 aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca
      241 tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg
      301 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
      361 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
      421 gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc accatgaaca
      481 ccccgggaat taacctgatc aaggaggaca tgcgcgtgaa ggtgcacatg gagggcaacg
      541 tgaacggcca cgccttcgtg atcgagggcg agggcaaggg caagccctac gagggcaccc
      601 agaccgccaa cctgaccgtg aaggagggcg cccccctgcc cttcagctac gacatcctga
      661 ccaccgccgt gcactacggc aaccgggtgt tcaccaagta ccccgaggac atccccgact
      721 acttcaagca gagcttcccc gagggctaca gctgggagcg caccatgacc ttcgaggaca
      781 agggcatctg caccatccgc agcgacatca gcctggaggg cgactgcttc ttccagaacg
      841 tgcgcttcaa gggcaccaac tt
//
