LOCUS       sequence-158545-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize
            using Asc1 and Not1 for mouse embryo injection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Mohr MA, Argast P, Pantazis P
  TITLE     Labeling cellular structures in vivo using confined primed 
            conversion of photoconvertible fluorescent proteins.
  JOURNAL   Nat Protoc. 2016 Dec;11(12):2419-2431. doi: 10.1038/nprot.2016.134. 
            Epub 2016 Nov 3.
  PUBMED    27809312
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2016.134"; journalName: "Nat Protoc"; date: 
            "2016-12"; volume: "11"; issue: "12"; pages: "2419-2431"
COMMENT     Sequence Label: p55-H2B-Dendra2
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        94..198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             383..761
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(417..436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
       61 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      121 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      181 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      241 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
      301 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
      361 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      421 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
      481 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      541 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
      601 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      661 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      721 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
      781 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
      841 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
      901 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg g
//
