LOCUS       sequence-158547-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y
  TITLE     WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 
            by DDB1-CUL4-ROC1 ligase.
  JOURNAL   Genes Dev. 2008 Apr 1. 22(7):866-71.
  PUBMED    18381890
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Apr 1. 22(7):866-71."
COMMENT     Sequence Label: pcDNA3-FLAG-DDB1
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             79..102
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacatgga ctacaaagac gatgacgata aaccaagctt ggtaccgggt
      121 atgtcgtaca actacgtggt aacggcccag aagcccaccg ccgtgaacgg ctgcgtgacc
      181 ggacacttta cttcggccga agacttaaac ctgttgattg ccaaaaacac gagattagag
      241 atctatgtgg tcaccgccga ggggcttcgg cccgtcaaag aggtgggcat gtatgggaag
      301 attgcggtca tggagctttt caggcccaag ggggagagca aggacctgct gtttatcttg
      361 acagcgaagt acaatgcctg catcctggag tataaacaga gtggcgagag cattgacatc
      421 attacgcgag cccatggcaa tgtccaggac cgcattggcc gcccctcaga gaccggcatt
      481 attggcatca ttgaccctga gtgccggatg attggcctgc gtctctatga tggccttttc
      541 aaggttattc cactagatcg cgataataaa gaactcaagg ccttcaacat ccgcctggag
      601 gagctgcatg tcattgatgt caagttccta tatggttgcc aagcacctac tatttgcttt
      661 gtctaccagg accctcaggg gcggcacgta aaaacctatg aggtgtctct ccgagaaaag
      721 gaattcaata agggcccttg gaaacaggaa aatgtcgaag ctgaagcttc catggtgatc
      781 gcagtcccag agccctttgg gggggccatc atcattggac aggagtcaat cacctatcac
      841 aatggtgaca aatacctggc
//
