LOCUS       sequence-158549-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral negative control vector containing scrambled 
            shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Sarbassov DD, Guertin DA, Ali SM, Sabatini DM
  TITLE     Phosphorylation and regulation of Akt/PKB by the rictor-mTOR 
            complex.
  JOURNAL   Science 2005 Feb 18;307(5712):1098-101.
  PUBMED    15718470
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2005-02-18"; volume: "307(5712)"; pages: "1098-101"
COMMENT     Sequence Label: scramble shRNA
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     124..143
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    299..416
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
       61 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
      121 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
      181 ttgtggaaag gacgaaacac cggtcctaag gttaagtcgc cctcgctcga gcgagggcga
      241 cttaacctta ggtttttgaa ttctcgacct cgagacaaat ggcagtattc atccacaatt
      301 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
      361 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
      421 tttattacag ggacagcaga gatccacttt ggccgcggct cgagggggtt ggggttgcgc
      481 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
      541 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
      601 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
      661 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
      721 actagtaccc tcgcagacgg acagcgccag gg
//
