LOCUS       sequence-158553-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-SunTag-PuroR
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(610..663)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(682..738)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     misc_feature    802..893
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(834..853)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    907..955
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 agcgggccct cacgctccag gcttcctcgt catgcaccaa gtgcgggggc cttcggggac
       61 ttccacgtcg gctgtcacgg tgaagcccaa ccgttcatag aagggaagat tccttggagc
      121 ggaggtctcc aagaaggcag gtactcctgc tctctcagct gcctccacgc cagggagcac
      181 tacggcagag cccagacctt tcccctgatg atcgggagat acaccgacgg ttgccaggaa
      241 ccaggcgggt tctttgggcc tgtgtggtgc cagcagtccc tccatttgct gctgtgccgc
      301 gagcctactc ccgctgagct cagccattct cgggccgatc tcagcgaata cggcgccagc
      361 ctcaactgat tcgggagtgg tccagacggc cacggcggcg ccgtcgtccg ccacccacac
      421 cttgcctatg tcgaggccaa ctcttgtaag aaacagctcc tgcagttcag tcactctctc
      481 aatatgccga tctgggtcaa cggtgtgtct cgtggcgggg tagtcggcga aggctgcagc
      541 cagagttctc acggctctag gaacgtcgtc tcttgtagcc aacctgacag taggcttgta
      601 ttcagtcatt gggccaggat tctcctcgac gtcaccgcat gttagcagac ttcctctgcc
      661 ctctcctccg gagccgctgc ccttcttcag tctggccacc tcgttctcca ggtggtagtt
      721 cttgctcagc agctcctcgg atccccctcc actgccacca gaccccccgc tttttgggta
      781 ctggcccgct agctatcatt aagagaaatg ttctggcacc tgcacttgca ctggggacag
      841 cctattttgc tagtttgttt tgtttcgttt tgttttgatg gagagcgtat gttagtacta
      901 tcgattcaca caaaaaacca acacacagat gtaatgaaaa taaagatatt ttattgcggc
      961 ctgtccaaaa ccctcccgta cc
//
