LOCUS       sequence-158554-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TagBFP-PuroR
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..759
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 tgatagctag cgggccagta cccaaaaagc ggggggtctg gtggcagtgg agggggatcc
       61 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
      121 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      181 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
      241 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
      301 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
      361 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
      421 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
      481 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
      541 gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc
      601 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
      661 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
      721 tactgcgacc tccctagcaa actggggcac aagcttaatt ccggaggaga gggcaga
//
