LOCUS       sequence-158556-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TagGFP2-PuroR
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..774
                     /codon_start=1
                     /product="monomeric green fluorescent protein, also known 
                     as mTagGFP"
                     /label=TagGFP2
                     /note="mammalian codon-optimized"
                     /translation="MSGGEELFAGIVPVLIELDGDVHGHKFSVRGEGEGDADYGKLEIK
                     FICTTGKLPVPWPTLVTTLCYGIQCFARYPEHMKMNDFFKSAMPEGYIQERTIQFQDDG
                     KYKTRGEVKFEGDTLVNRIELKGKDFKEDGNILGHKLEYSFNSHNVYIRPDKANNGLEA
                     NFKTRHNIEGGGVQLADHYQTNVPLGDGPVLIPINHYLSTQTKISKDRNEARDHMVLLE
                     SFSACCHTHGMDELYR"
ORIGIN
        1 tgatagctag cgggccagta cccaaaaagc ggggggtctg gtggcagtgg agggggatcc
       61 atgagcgggg gcgaggagct gttcgccggc atcgtgcccg tgctgatcga gctggacggc
      121 gacgtgcacg gccacaagtt cagcgtgcgc ggcgagggcg agggcgacgc cgactacggc
      181 aagctggaga tcaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctg
      241 gtgaccaccc tctgctacgg catccagtgc ttcgcccgct accccgagca catgaagatg
      301 aacgacttct tcaagagcgc catgcccgag ggctacatcc aggagcgcac catccagttc
      361 caggacgacg gcaagtacaa gacccgcggc gaggtgaagt tcgagggcga caccctggtg
      421 aaccgcatcg agctgaaggg caaggacttc aaggaggacg gcaacatcct gggccacaag
      481 ctggagtaca gcttcaacag ccacaacgtg tacatccgcc ccgacaaggc caacaacggc
      541 ctggaggcta acttcaagac ccgccacaac atcgagggcg gcggcgtgca gctggccgac
      601 cactaccaga ccaacgtgcc cctgggcgac ggccccgtgc tgatccccat caaccactac
      661 ctgagcactc agaccaagat cagcaaggac cgcaacgagg cccgcgacca catggtgctc
      721 ctggagtcct tcagcgcctg ctgccacacc cacggcatgg acgagctgta caggtccgga
      781 ggagagggca ga
//
