LOCUS       sequence-158557-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TagGFP2-PuroR
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(613..666)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tgaagcgggc cctcacgctc caggcttcct cgtcatgcac caagtgcggg ggccttcggg
       61 gacttccacg tcggctgtca cggtgaagcc caaccgttca tagaagggaa gattccttgg
      121 agcggaggtc tccaagaagg caggtactcc tgctctctca gctgcctcca cgccagggag
      181 cactacggca gagcccagac ctttcccctg atgatcggga gatacaccga cggttgccag
      241 gaaccaggcg ggttctttgg gcctgtgtgg tgccagcagt ccctccattt gctgctgtgc
      301 cgcgagccta ctcccgctga gctcagccat tctcgggccg atctcagcga atacggcgcc
      361 agcctcaact gattcgggag tggtccagac ggccacggcg gcgccgtcgt ccgccaccca
      421 caccttgcct atgtcgaggc caactcttgt aagaaacagc tcctgcagtt cagtcactct
      481 ctcaatatgc cgatctgggt caacggtgtg tctcgtggcg gggtagtcgg cgaaggctgc
      541 agccagagtt ctcacggctc taggaacgtc gtctcttgta gccaacctga cagtaggctt
      601 gtattcagtc attgggccag gattctcctc gacgtcaccg catgttagca gacttcctct
      661 gccctctcct ccggacctgt acagctcgtc catgccgtgg gtgtggcagc aggcgctgaa
      721 ggactccagg agcaccatgt ggtcgcgggc ctcgttgcgg tccttgctga tcttggtctg
      781 agtgctcagg tagtggttga tggggatcag cacggggccg tcgcccaggg gcacgttggt
      841 ctggtagtgg tcggccagct gcacgccgcc gccctcgatg ttgtggcgg
//
