LOCUS       sequence-158558-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TagRFP-PuroR
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..771
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 tgatagctag cgggccagta cccaaaaagc ggggggtctg gtggcagtgg agggggatcc
       61 atggtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct gtacatggag
      121 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
      181 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
      241 atcctggcta ccagcttcat gtacggcagc agaaccttca tcaaccacac ccagggcatc
      301 cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
      361 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
      421 tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
      481 acactcggct gggaggccaa caccgagatg ctgtaccccg ctgacggcgg cctggaaggc
      541 agaagcgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttcaagacc
      601 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac
      661 cacagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg
      721 gctgtggcca gatactgcga cctccctagc aaactggggc acaaacttaa ttccggagga
      781 gagggcag
//
