LOCUS       sequence-158559-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TagRFP-PuroR
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(609..662)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gcgggccctc acgctccagg cttcctcgtc atgcaccaag tgcgggggcc ttcggggact
       61 tccacgtcgg ctgtcacggt gaagcccaac cgttcataga agggaagatt ccttggagcg
      121 gaggtctcca agaaggcagg tactcctgct ctctcagctg cctccacgcc agggagcact
      181 acggcagagc ccagaccttt cccctgatga tcgggagata caccgacggt tgccaggaac
      241 caggcgggtt ctttgggcct gtgtggtgcc agcagtccct ccatttgctg ctgtgccgcg
      301 agcctactcc cgctgagctc agccattctc gggccgatct cagcgaatac ggcgccagcc
      361 tcaactgatt cgggagtggt ccagacggcc acggcggcgc cgtcgtccgc cacccacacc
      421 ttgcctatgt cgaggccaac tcttgtaaga aacagctcct gcagttcagt cactctctca
      481 atatgccgat ctgggtcaac ggtgtgtctc gtggcggggt agtcggcgaa ggctgcagcc
      541 agagttctca cggctctagg aacgtcgtct cttgtagcca acctgacagt aggcttgtat
      601 tcagtcattg ggccaggatt ctcctcgacg tcaccgcatg ttagcagact tcctctgccc
      661 tctcctccgg aattaagttt gtgccccagt ttgctaggga ggtcgcagta tctggccaca
      721 gccacctcgt gctgctcgac gtaggtctct ttgtcggcct ccttgattct ttccagtctg
      781 tggtccacat agtagacgcc gggcatcttg aggttcttag cgggtttctt ggatctgtat
      841 gtggtcttga agttgcagat caggtggccc ccgcccacga gcttcagggc catgtcgctt
      901 ctgccttcca ggccgccgtc agcggggtac agcatctcgg tgttggcctc ccagccgagt
//
