LOCUS       sequence-158560-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TC-PuroR
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..78
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             88..141
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tgatagctag cgggccagta cccaaaaagc ggggggtctg gtggcagtgg agggggatcc
       61 tgctgccccg gctgctgctc cggaggagag ggcagaggaa gtctgctaac atgcggtgac
      121 gtcgaggaga atcctggccc aatgactgaa tacaagccta ctgtcaggtt ggctacaaga
      181 gacgacgttc ctagagccgt gagaactctg gctgcagcct tcgccgacta ccccgccacg
      241 agacacaccg ttgacccaga tcggcatatt gagagagtga ctgaactgca ggagctgttt
      301 cttacaagag ttggcctcga cataggcaag gtgtgggtgg cggacgacgg cgccgccgtg
      361 gccgtctgga ccactcccga atcagttgag gctggcgccg tattcgctga gatcggcccg
      421 agaatggctg agctcagcgg gagtaggctc gcggcacagc agcaaatgga gggactgctg
      481 gcaccacaca ggcccaaaga acccgcctgg ttcctggcaa ccgtcggtgt atctcccgat
      541 catcagggga aaggtctggg ctctgccgta gtgctccctg gcgtggaggc agctgagaga
      601 gcaggagtac ctgccttctt ggagacctcc gctccaagga atcttccctt ctatgaacgg
      661 ttgggcttca ccgtgacagc cgacgtggaa gtccccgaag gcccccgcac ttggtgcatg
      721 acgaggaagc ctggagcgtg agggcccgct tcgagcagac atgataagat acattgatga
      781 gtttggac
//
