LOCUS       sequence-158561-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-TC-PuroR
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(612..665)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(675..692)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     misc_feature    756..847
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(788..807)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    861..909
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gaagcgggcc ctcacgctcc aggcttcctc gtcatgcacc aagtgcgggg gccttcgggg
       61 acttccacgt cggctgtcac ggtgaagccc aaccgttcat agaagggaag attccttgga
      121 gcggaggtct ccaagaaggc aggtactcct gctctctcag ctgcctccac gccagggagc
      181 actacggcag agcccagacc tttcccctga tgatcgggag atacaccgac ggttgccagg
      241 aaccaggcgg gttctttggg cctgtgtggt gccagcagtc cctccatttg ctgctgtgcc
      301 gcgagcctac tcccgctgag ctcagccatt ctcgggccga tctcagcgaa tacggcgcca
      361 gcctcaactg attcgggagt ggtccagacg gccacggcgg cgccgtcgtc cgccacccac
      421 accttgccta tgtcgaggcc aactcttgta agaaacagct cctgcagttc agtcactctc
      481 tcaatatgcc gatctgggtc aacggtgtgt ctcgtggcgg ggtagtcggc gaaggctgca
      541 gccagagttc tcacggctct aggaacgtcg tctcttgtag ccaacctgac agtaggcttg
      601 tattcagtca ttgggccagg attctcctcg acgtcaccgc atgttagcag acttcctctg
      661 ccctctcctc cggagcagca gccggggcag caggatcccc ctccactgcc accagacccc
      721 ccgctttttg ggtactggcc cgctagctat cattaagaga aatgttctgg cacctgcact
      781 tgcactgggg acagcctatt ttgctagttt gttttgtttc gttttgtttt gatggagagc
      841 gtatgttagt actatcgatt cacacaaaaa accaacacac agatgtaatg aaaataaaga
      901 tattttattg cggcctgtcc aatacctccc gtaccttaat attacttact tatccttgaa
      961 agacgtacta gtaaccctga taaatgcttc ataatattga aaaag
//
