LOCUS       sequence-158562-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-3xflag-PuroR
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..138
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             148..201
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 catttctctt aatgatagct agcgggccag tacccaaaaa gcggggggtc tggtggcagt
       61 ggagggggat ccgattacaa ggaccacgac ggcgattata aggaccatga tattgattat
      121 aaggacgatg acgataagtc cggaggagag ggcagaggaa gtctgctaac atgcggtgac
      181 gtcgaggaga atcctggccc aatgactgaa tacaagccta ctgtcaggtt ggctacaaga
      241 gacgacgttc ctagagccgt gagaactctg gctgcagcct tcgccgacta ccccgccacg
      301 agacacaccg ttgacccaga tcggcatatt gagagagtga ctgaactgca ggagctgttt
      361 cttacaagag ttggcctcga cataggcaag gtgtgggtgg cggacgacgg cgccgccgtg
      421 gccgtctgga ccactcccga atcagttgag gctggcgccg tattcgctga gatcggcccg
      481 agaatggctg agctcagcgg gagtaggctc gcggcacagc agcaaatgga gggactgctg
      541 gcaccacaca ggcccaaaga acccgcctgg ttcctggcaa ccgtcggtgt atctcccgat
      601 catcagggga aaggtctggg ctctgccgta gtgctccctg gcgtggaggc agctgagaga
      661 gcaggagtac ctgccttctt ggagacctcc gctccaagga atcttccctt ctatgaacgg
      721 ttgggcttca ccgtgacagc cgacgtggaa gtccccgaag gcccccgcac ttggtgcatg
      781 acgaggaagc ctgg
//
