LOCUS       sequence-158564-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9, mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pRZ-CAS9-mCherry
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             265..318
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
       61 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      121 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
      181 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
      241 gtcgctacca tcgagggatc cggagagggc agaggaagtc tgctaacatg cggtgacgtc
      301 gaggagaatc ctggcccaat ggacaagaag tacagcatcg gcctggacat cggcaccaac
      361 agcgtgggct gggccgtgat caccgacgag tacaaggtgc ccagcaagaa gttcaaggtg
      421 ctgggcaaca ccgaccgcca cagcatcaag aagaacctga tcggcgccct gctgttcgac
      481 agcggcgaga ccgccgaggc cacccgcctg aagcgcaccg cccgccgccg ctacacccgc
      541 cgcaagaacc gcatctgcta cctgcaggag atcttcagca acgagatggc caaggtggac
      601 gacagcttct tccaccgcct ggaggagagc ttcctggtgg aggaggacaa gaagcacgag
      661 cgccacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc
      721 atctaccacc tgcgcaagaa gctggtggac agcaccgaca a
//
