LOCUS       sequence-158565-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9, mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pRZ-CAS9-mCherry
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             284..304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcgcgtgatc ctggccgacg ccaacctgga caaggtgctg agcgcctaca acaagcaccg
       61 cgacaagccc atccgcgagc aggccgagaa catcatccac ctgttcaccc tgaccaacct
      121 gggcgccccc gccgccttca agtacttcga caccaccatc gaccgcaagc gctacaccag
      181 caccaaggag gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac
      241 ccgcatcgac ctgagccagc tgggcggcga cagccgcgcc gaccccaaga agaagcgcaa
      301 ggtgtgagcg gccgcatcga taaaataaaa gattttattt agtctccaga aaaagggggg
      361 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
      421 atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac
      481 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      541 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      601 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
      661 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
      721 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
      781 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
      841 ggtacccgtg tatccaataa a
//
