LOCUS       sequence-158568-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9, TagBFP, and sgRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-BFP empty
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             222..275
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga
       61 aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa
      121 tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact
      181 gcgacctccc tagcaaactg gggcacaagc taaattccgg agagggcaga ggaagtctgc
      241 taacatgcgg tgacgtcgag gagaatcctg gcccaatgga caagaagtac agcatcggcc
      301 tggacatcgg caccaacagc gtgggctggg ccgtgatcac cgacgagtac aaggtgccca
      361 gcaagaagtt caaggtgctg ggcaacaccg accgccacag catcaagaag aacctgatcg
      421 gcgccctgct gttcgacagc ggcgagaccg ccgaggccac ccgcctgaag cgcaccgccc
      481 gccgccgcta cacccgccgc aagaaccgca tctgctacct gcaggagatc ttcagcaacg
      541 agatggccaa ggtggacgac agcttcttcc accgcctgga ggagagcttc ctggtggagg
      601 aggacaagaa gcacgagcgc caccccatct tcggcaacat cgtggacgag gtggcctacc
      661 acgagaagta ccccaccatc taccacctgc gcaagaagct ggtggacagc accgacaagg
      721 ccgacctgcg cctgatctac ctggccctgg cccacatgat caagttccgc ggccacttcc
      781 tgatcgaggg cgacctgaac cccgacaaca gcgacgt
//
