LOCUS       sequence-158569-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9, TagBFP, and sgRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-BFP empty
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     129..148
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        305..684
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        685..888
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     839..859
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atcaggctgt tagagagata attggaatta atttgactgt aaacacaaag atattagtac
       61 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
      121 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
      181 atatcttgtg gaaaggacga aacaccgggc cctcgatatc gactagtccg ttatcaactt
      241 gaaaaagtgg caccgagtcg gtgctttttt tcgaattccg atgtacgggc cagatatacg
      301 cgttgacatt gattattgtc tagttattaa tagtaatcaa ttacggggtc attagttcat
      361 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      421 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      481 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      541 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      601 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      661 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      721 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      781 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      841 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      901 agagaaccca ctgcttactg gcttatcgaa attaagcttc ccatgagcga gctgatta
//
