LOCUS       sequence-158575-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea 
            asigna virus peptide (2A), and mCherry protein; flanked by Tol2 
            sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-ubb-cytoBirA-2a-mCherry
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..748)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(316..335)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(755..808)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 agcggccggc cgccccgact ctagagtcgc ggccgcttta cttgtacagc tcgtccatgc
       61 cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt
      121 tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct
      181 tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca
      241 gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg
      301 cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc
      361 gcagcttcac tttataaatg aactcgccat cttggaggga ggagtcctgg gtcacggtca
      421 ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca
      481 gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtacatga
      541 attgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc actttaagct
      601 tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt
      661 ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg atgatggcca
      721 tgttatcctc ctcgcccttc gacaccatac ttccgggccc gggattttcc tccacgtccc
      781 cgcatgttag aagacttccc ctgccctctc ctgatccgat atctttttct gcactacgca
      841 gggatatttc accgcccatc cagggtttta ttattccatc ctgctcaagt aataaagccc
      901 cctgtttgtc tattccgcgt gaaatgccaa atatttcttt atcaccaatg ataagtttca
      961 ctgggcgatt aata
//
