LOCUS       sequence-158576-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral backbone for cloning and expression of new shRNA 
            sequences. Uses puromycin for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2003-04"; volume: "9(4)"; pages: "493-501"
COMMENT     Sequence Label: pLKO.1 puro
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    124..241
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(304..552)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(362..381)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(532..552)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(850..894)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg
       61 cgcaacccca accccctcga gccgcggcca aagtggatct ctgctgtccc tgtaataaac
      121 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
      181 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
      241 attgtggatg aatactgcca tttgtctcga ggtcgagaat tcacgcgtgc atacctgcgg
      301 accggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt
      361 tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag
      421 aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt
      481 aattccaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg
      541 aaataggccc tcggtgaagg gggcggccgc tcgaggctag tctcgtgatc gataccgtcg
      601 agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat
      661 tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga
      721 atatccctgc ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac
      781 caagcctcct actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa
      841 ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg
      901 ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt t
//
