LOCUS       sequence-158577-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea 
            asigna virus peptide (2A), and mCherry protein; flanked by Tol2 
            sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-ubb-cytoBirA-2a-mCherry
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(221..247)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(254..280)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(287..313)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     regulatory      323..332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cattaagtaa ctggataggc tcaggcaggc tgtatccttt tcccggaacg gtaaagacat
       61 caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg ctcattccca
      121 gcgtttcacc caactgctcg ccagagtgaa attcaccgtt cgctaacagg gcaatcaatt
      181 tcagtggcac ggtgttatcc ttcataggag atctgcctgg aagtgaggca tagtcgggca
      241 catcgtaacc cgggaggcta gcataatcag gaacatcata tccgggcaaa cttgcgtaat
      301 ctgggacgtc ataggtggcc atccatggtg gcggatccct gtaaacaaat tcaaagtaag
      361 attagcaatc acacatttcc atttaaaaaa tactacaatg ttctagccaa taatgtatgt
      421 tttgcacttc tcccatgtta aacgagatag ttcacccgaa aatgaaattt tactttgcat
      481 ttggtcaccc ttaaatgtta caaaccttta aaataataga aaactattga ctatacttgt
      541 agagaaaaat aaatactatc caaaacaatg gctgacagtg gactatagaa tgcattttac
      601 ctgcagcaaa attgtccaaa tgatcctata tgaaaattta gggtcttacc tagtgaaatg
      661 atagctcatt ttgcacaagc tctgagattt ggctttgtgc atcatgaaat ctaattatgt
      721 catgcatttc caaagcattg gccattttaa acaaactgca catctgtgca gtttacattg
      781 ttttaaaata aaaaataaaa aattaagaaa taaagaataa taatgttcca caacattaca
      841 ttaggtagca gattggcaga gaaccaaagg gtggggccag taaggaagga cttctacaca
      901 gtaaggaaac gctgaaattc aacttaatca ctcagtcatt taaaaataaa tacttttatt
      961 ttttttattt ttaaag
//
