LOCUS       sequence-158579-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with 
            nuclear localization signal (NLS), a Thosea asigna virus peptide 
            (2A), and mCherry protein; flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-ubb-NLS-BirA-2a-mCherry
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(212..232)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(248..274)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(281..307)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(314..340)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     regulatory      350..359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cattaagtaa ctggataggc tcaggcaggc tgtatccttt tcccggaacg gtaaagacat
       61 caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg ctcattccca
      121 gcgtttcacc caactgctcg ccagagtgaa attcaccgtt cgctaacagg gcaatcaatt
      181 tcagtggcac ggtgttatcc ttcatagatc gtacttttct cttctttttt ggaggagatc
      241 tgcctggaag tgaggcatag tcgggcacat cgtaacccgg gaggctagca taatcaggaa
      301 catcatatcc gggcaaactt gcgtaatctg ggacgtcata ggtggccatc catggtggcg
      361 gatccctgta aacaaattca aagtaagatt agcaatcaca catttccatt taaaaaatac
      421 tacaatgttc tagccaataa tgtatgtttt gcacttctcc catgttaaac gagatagttc
      481 acccgaaaat gaaattttac tttgcatttg gtcaccctta aatgttacaa acctttaaaa
      541 taatagaaaa ctattgacta tacttgtaga gaaaaataaa tactatccaa aacaatggct
      601 gacagtggac tatagaatgc attttacctg cagcaaaatt gtccaaatga tcctatatga
      661 aaatttaggg tcttacctag tgaaatgata gctcattttg cacaagctct gagatttggc
      721 tttgtgcatc atgaaatcta attatgtcat gcatttccaa agcattggcc attttaaaca
      781 aactgcacat ctgtgcagtt tacattgttt taaaataaaa aataaaaaat taagaaataa
      841 agaataataa tgttccacaa cattacatta ggtagcagat tggcagagaa ccaaagggtg
      901 gggccagtaa ggaaggactt ctacacagta aggaaacgct gaaattcaac ttaatcactc
      961 agtcatttaa aaataaatac ttttattttt tttat
//
