LOCUS       sequence-158597-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacillus subtilis sgRNA expression vector; integrates into thrC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins 
            JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, 
            Brown ED, Qi LS, Huang KC, Gross CA
  TITLE     A Comprehensive, CRISPR-based Functional Analysis of Essential Genes
            in Bacteria.
  JOURNAL   Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. 
            Epub 2016 May 26.
  PUBMED    27238023
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.003"; journalName: "Cell"; date: "2016-06-2-
            2"; volume: "165"; issue: "6"; pages: "1493-506"
COMMENT     Sequence Label: pJMP3
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     107..124
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(180..197)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(213..242)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_RNA        complement(263..338)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga ccaccgcgct
       61 actgccgcca ggcaaattct gttttatcag accgcttctg cgttctgatt taatctgtat
      121 caggctgaaa atcttctctc atccgccaaa acagccaagc tggagaccgt ttaaactcaa
      181 tgatgatgat gatgatggtc gacggcgcta ttcagatcct cttctgagat gagtttttgt
      241 tcgggcccaa gcttcaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga
      301 ctagccttat tttaacttgc tatttctagc tctaaaacag accgctaaac tgaaagttac
      361 atttattgta caacacgagc ccatttttgt caaataaaat ttaaattata tcaacgttaa
      421 taaggaattc gataagcttc taggatccga aggcagcagt tttttggcct tcaatgcggt
      481 aagggttgac tgagttgaca agggcaatcg gtgatttctc acagatggaa cggacaattt
      541 taagcgcatc gtcaaagttt ccgtcaattg cgataatctc ggctccgtac atgacagctt
      601 gagcgagttt tccaaatgca atttttccgt tcgggatgat gacaatgcat ttcatgttag
      661 cacgggctgc atatgctgcc gcagcagcgg aagtgttacc tgttgacgcg cacataatcg
      721 tgtcattgcc ttcttctttt gcctttgcca cagccataac cattccgcga tctttaaatg
      781 atcccgtagg attgacgcct tccgttttga catgaagctc aattccgagc tgctcagaca
      841 gcttcggcag gtgaataaga ggtgtgtttc cttcatgtaa agttagcgcc ggtgtttgat
      901 ctgttacagg taaaaattct ttatattgat ggataagtcc tttccacatt agctccaacc
      961 gttcccttct acacgatatg tgcttttgac ttcttgaacg acttccaaat catt
//
