LOCUS       sequence-158600-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains pBAD-CusR and pCusR-antiscaffold with no fluorescent 
            protein. Used as a control for autofluorescence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH004
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(40..69)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(418..445)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(461..532)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aactcaatga tgatgatgat gatggtcgac ggcgctattc agatcctctt ctgagatgag
       61 tttttgttcg ggcccaagct tctcactatt tgtaatactt ctccacgtaa gggacaggaa
      121 tcatccccct ctttccttcg ctgtcctctg cattccacca ctgctcttca ggcttatccc
      181 ggattctcag gatgtctcct ttcttaaagg gaaggtcttc ttcatcattc ccattaaagt
      241 caaacagggc ccgcacatac tctgccatct aatcctcctg cgcccgtttc ttacgaatgt
      301 atgagctcat aatttctggt gattttatgc cgccaacttt actcgccagg ctctgatttt
      361 ccggtgacag aaaaatgaca aaattgtcat tttgccaatc ctaggccgcg gtataaacgc
      421 agaaaggccc acccgaaggt gagccagtgt gactctagta gagagcgttc accgacaaac
      481 aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg
      541 ctcgagtcag gatccttaaa gcgggccgta tcgggtttcg tatttggata caatgttgcg
      601 gcaccggccc acacgtttac ggagttcggc cgcgcgggta cgcagtgcgg tgttttcttt
      661 ttcaagaaac gctgcgcgga tttccagaga tccaccgctg ccggacccag atccctgacc
      721 atccggcacc tcaagcatgt aacccacgcc gcgcacggtc tgaattagct tcggctcaaa
      781 gtcgttgtcg atttt
//
