LOCUS       sequence-158604-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector to knocin EGFP into human Brachyury(T) locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Chu LF, Leng N, Zhang J, Hou Z, Mamott D, Vereide DT, Choi J, 
            Kendziorski C, Stewart R, Thomson JA
  TITLE     Single-cell RNA-seq reveals novel regulators of human embryonic stem
            cell differentiation to definitive endoderm.
  JOURNAL   Genome Biol. 2016 Aug 17;17(1):173. doi: 10.1186/s13059-016-1033-x.
  PUBMED    27534536
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2016-08-17"; volume: "17(1):173. doi"; pages: " 
            10.1186/s13059-016-1033-x"
COMMENT     Sequence Label: T-2A-EGFP-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             528..584
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(630..651)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcttggtacc gagctcggat cccatcttcc tgatgatttt gtttcttatt aatagatacg
       61 acaataactt ctatacatca cagaatgtgc ttgtgagtcc tttaataatt tggagaaaat
      121 gacctgtttg aaagaagaaa actgtcatat tacacagtca ctccgaatgg gatttctggt
      181 gtgtttttcc atccttagct ggcctgcagc ccctgcccag gccctgctca ctggtgtctt
      241 tctgttgcag tcagtacccc agcctgtggt ctgtgagcaa cggcgccgtc accccgggct
      301 cccaggcagc agccgtgtcc aacgggctgg gggcccagtt cttccggggc tcccccgcgc
      361 actacacacc cctcacccat ccggtctcgg cgccctcttc ctcgggatcc ccactgtacg
      421 aaggggcggc cgcggccaca gacatcgtgg acagccagta cgacgccgca gcccaaggcc
      481 gcctcatagc ctcatggaca cctgtgtcgc caccttccat ggctagcgcc actaacttct
      541 ccctgttgaa acaagcaggg gatgtcgaag agaatcccgg gccaatggtg agcaagggcg
      601 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      661 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      721 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      781 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      841 agtcc
//
