LOCUS       sequence-158605-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector to knocin EGFP into human Brachyury(T) locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Chu LF, Leng N, Zhang J, Hou Z, Mamott D, Vereide DT, Choi J, 
            Kendziorski C, Stewart R, Thomson JA
  TITLE     Single-cell RNA-seq reveals novel regulators of human embryonic stem
            cell differentiation to definitive endoderm.
  JOURNAL   Genome Biol. 2016 Aug 17;17(1):173. doi: 10.1186/s13059-016-1033-x.
  PUBMED    27534536
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2016-08-17"; volume: "17(1):173. doi"; pages: " 
            10.1186/s13059-016-1033-x"
COMMENT     Sequence Label: T-2A-EGFP-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    109..142
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(734..753)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(735..753)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cctgaagaac gagatcagca gcctctgttc cacatacact tcattctcag tattgttttg
       61 ccaagttcta attccatcag aagctggtcg agatccggaa cccttaatat aacttcgtat
      121 aatgtatgct atacgaagtt attaggtccc tcgaagaggt tcactaggcg cgccagcagc
      181 aaggcccagg tcccgaaaga tgcagtgact ttttgtcgtg gcagccagtg gtgactggat
      241 tgacctacta ggtacccagt ggcagtctca ggttaagaag gaaatgcagc ctcagtaact
      301 tccttttcaa agcagtggag gagcacacgg cacctttccc cagagcccca gcatcccttg
      361 ctcacacctg cagtagcggt gctgtcccag gtggcttaca gatgaaccca actgtggaga
      421 tgatgcagtt ggcccaacct cactgacggt gaaaaaatgt ttgccagggt ccagaaactt
      481 tttttggttt atttctcata cagtgtattg gcaactttgg cacaccagaa tttgtaaact
      541 ccaccagtcc tactttagtg agataaaaag cacactctta atcttcttcc ttgttgcttt
      601 caagtagtta gagttgagct gttaaggaca gaataaaatc atagttgagg acagggccgg
      661 ccaaaagggc gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag
      721 agggcccaat tcgccctata gtgagtcgta ttacaattca c
//
