LOCUS       sequence-158612-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used for abrB0310(CAC0310) overexpression in Clostridium 
            acetobutylicum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Xue Q, Yang Y, Chen J, Chen L, Yang S, Jiang W, Gu Y
  TITLE     Roles of three AbrBs in regulating two-phase Clostridium 
            acetobutylicum fermentation.
  JOURNAL   Appl Microbiol Biotechnol. 2016 Jun 8.
  PUBMED    27276910
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol. 2016 Jun 8."
COMMENT     Sequence Label: pIMP1-Pthl-AbrB0310
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    338..359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(374..379,380..397,398..404)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    412..428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     417..439
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     436..452
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     436..452
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      241 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
      301 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      361 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      421 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt tggctaacac
      481 acacgccatt ccaaccaata gttttctcgg cataaagcca tgctctgacg cttaaatgca
      541 ctaatgcctt aaaaaaacat taaagtctaa cacactagac ttatttactt cgtaattaag
      601 tcgttaaacc gtgtgctcta cgaccaaaag tataaaacct ttaagaactt tcttttttct
      661 tgtaaaaaaa gaaactagat aaatctctca tatcttttat tcaataatcg catcagattg
      721 cagtataaat ttaacgatca ctcatcatgt tcatatttat cagagctcgt gctataatta
      781 tactaatttt ataaggagga aaaaataaag agggttataa tgaacgagaa aaatataaaa
      841 cacagtcaaa actttattac ttcaaaacat aatatagata aaataatgac aaatataaga
      901 ttaaatgaac atgataatat ct
//
